针刺结合运动训练干预脑梗死大鼠海马CA3区微管相关蛋白及突触素表达及学习记忆功能变化

实验观察针刺百会(GV20)、曲鬓(GB 7)和前顶穴(GV21)联合运动训练干预和件下大脑中动脉闭塞大鼠模型大鼠海马CA3区微管相关蛋白2和突触素蛋白的表达，并和单独运动训练干预的大鼠对比。Y型迷宫实验和免疫组织化学染色显示，建模后5周，针刺联合运动训练组穿越Y型迷宫错误次数均少于模型组和运动训练组，针刺联合运动训练组的微管相关蛋白2和突触素表达明显增高，各组海马CA3区的微管相关蛋白2，突触素的表达和穿越Y型迷宫错误次数均呈明显的负相关性。说明针刺联合运动训练可以改善脑梗死大鼠学习和记忆功能，其机制与海马CA3区的树突及突触可塑性有关。

Influence of acupuncture with exercise training on learning and memory functions, as well as microtubule-associated protein-2 and synaptophysin expression in the hippocampal CA3 region, in a rat model of cerebral infarction

The present study was designed to determine microtubule-associated protein-2 and synaptophysin expression in the hippocampal CA3 region in a rat model of middle cerebral artery occlusion. The rats were treated with acupuncture at Baihui (GV 20), Qubin (GB 7), and Qianding (GV 21) points, in addition to exercise training. Results were compared with rats undergoing exercise training only. The Y-maze method and immunohistochemistry revealed decreased error frequency of passing through Y-maze, as well as significantly increased microtubule-associated protein-2 and synaptophysin expression, in the acupuncture with exercise training group compared with the model and exercise training groups after 5 weeks. Microtubule-associated protein-2 and synaptophysin expressions negatively correlated with error frequency of passing through the Y-maze. These results suggested that acupuncture combined with exercise training improved learning and memory functions in a rat model of cerebral infarction. The mechanisms of action were hypothesized to be associated with dendritic or synaptic plasticity in the ipsilateral hippocampal CA3 region.