慢性丙型肝炎患者淋巴细胞亚群及其凋亡研究

目的 探讨ＨＣＶ感染诱导自身免疫反应的机理。方法 应用ＥＬＩＳＡ和流式细胞术检测了１８例慢性丙型肝炎患者及１６例正常人血清丙型肝炎病毒感染相关自身抗体（抗ＧＯＲ）及抗核抗体（ＡＮＡ）、外周血淋巴细胞（ＰＢＭＣ）亚群及其凋亡百分率。结果 慢性丙型肝炎患者血清抗ＧＯＲ抗体及ＡＮＡ阳性率分别为６６．７％和４４．４％，与正常对照组比较差异均有显著意义（Ｘ＾２＝２６．８６、Ｘ＾２＝９．３０，Ｐ〈０．００５）

Lymphocyte subset and its apoptosis in chronic hepatitis C

Objective To explore the autoimmune pathogenesis induced by hepatitis C virus. Methods Serumanti- GOR and peripheral blood lymphocyte subset and its apoptosis rate atier activated by Pokeweed mitogen (pwm ) in vitrowere measured by enzyme-linked immunosorbent assay and flow cytometry , respectively. Eighteen individuals with chronichepatitis C and 16 healthy controls were obserxed. Results The positive rates of serum anti-GOR (66.7% ) and ANA (44.4% )were significantly higher in chronic hepatitis C than normal controls (x~ 2 = 26.86. x~ 2 = 9.30. P < 0.005 ). The apoptosis per-centage of PBMCs and CD_3~ T cell was elevated in individuals with chronic hepatitis C when compared with normal con-trols (t = 2.44. P < 0.05 ). However. the apoptosis percentage of CD_4~ T and CD _19~ B cells in PBMCs was significantly de-creased in patients with anti-GOR positive compared to anti-GOR negative (t = 3. 1 7. P < 0.01; t = 2.294. P <0.05 ). Similarly.the percentage of CD_4~ CD_8~ T cells in anti-GOR positive patients was increased and CD_3~ /CD_4~ was decreased (t = 2. 1 8. P<0.05) compared to anti-GOR negative (t = 2.44. P < 0.05; t = 2. 18. P <0.05 ). Conclusion Autoimmune response exists inchronic hepatitis C patients. There is an imbalance of lymphocyte subset and its apoptosis, which may be one of the impor-tant reasons for the mechanism of autoimmune pathogenesis of chronic hepatitis C.[