Envelope proteins of vesicular stomatitis virions: accessibility to iodination |
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Authors: | N F Moore J M Kelley R R Wagner |
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Affiliation: | Department of Microbiology, The University of Virginia School of Medicine, Charlottesville, Virginia 22901 USA |
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Abstract: | The glycoprotein and, to a lesser extent, the matrix membrane protein of intact vesicular stomatitis virions were specificially iodinated by oxidation with lactoperoxidase or chloramine T. The virion envelope provided an effective barrier against iodination of nucleocapsid proteins. Selective removal of glycoprotein by trypsin or Triton X-100 exposed the membrane matrix protein to somewhat more extensive iodination but the nucleocapsid N protein became only slightly more accessible to iodination; the nucleocapsid L and NS proteins remained unlabeled. |
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