Analysis of human growth hormone-binding protein in plasma by high pressure liquid chromatography] |
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| Authors: | A Tar J F Hocquette J C Souberbielle J P Clot R Brauner M C Postel-Vinay |
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| Affiliation: | INSERM U.30, H?pital des Enfants-Malades, Paris. |
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| Abstract: | A technique using high pressure liquid chromatography gel filtration was used to evaluate GH-binding proteins (GH-BPs) in human plasma; eluate was monitored for radioactivity in a gamma-detection system connected to a computer. Plasma (200 microliters) was incubated with 125I-human (h) GH (200,000 cpm) at 4 degrees C for 20 hours. Our GH binding assay offers important gains in terms of rapidity and resolution; it has permitted a clear separation and characterization of the two GH-binding components present in human plasma. |
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