中药多糖在信号转导中诱导细胞凋亡的探讨

 目的 研究中药多糖在信号转导中诱导细胞凋亡的作用并探讨其作用机制。方法 培养1、8h后，经流式细胞仪检测细胞凋亡情况。结果 茯苓多糖、黄芪多糖、猪苓多糖诱导S180肉瘤细胞的凋亡，其效果与茯苓多糖、黄芪多糖、猪苓多糖的浓度和作用时间有关。实验组培养液，分别加入茯苓多糖、黄芪多糖、猪苓多糖各100μg／mL、250μg／mL和500μg／mL，培养1、8h后细胞凋亡率有增高趋势。实验组细胞凋亡率与对照组相比有显著性差异（P〈0．05）。结论 中药多糖在早期能诱导细胞凋亡，在晚期能引起细胞坏死，抑制S180肉瘤细胞的增殖。

Study of the Cell Apoptosis and Mechanism of Plant Polysaccharide in Cellular Signalling Pathway

Objective 　To study the cell apoptosis and probe its under lying mechanism of plant polysaccharide in cellular signalling pathway. Methods 　After cultured for 1 and 8h , the cell apoptotic rate of mice sarcoma S-₁₈₀ ell line was observed by flow cytomet ry. Results 　cell apoptosis of mice sarcoma S- ₁₈₀ cell line was induced by PB (polyporusum bellatus) PPS (pachyman polysaccharides) APS (ast ragalus polysaccharides) in dose-and time-dependent manners. Af ter respectably treated with 100 ,250 ,500μg/ mL of Plant polysaccharide for 1 and 8h. The cell apoptotic rate of mice sarcoma S- ₁₈₀ cell line were on the rise with time and dose. The apoptotic rate of experiment groups have significant difference compared with control ( P < 0. 05) . Conclusion 　Plant polysaccharide may induce cell apoptosis of S- ₁₈₀ cell line in early phase and induce necrocytosis in late phase ,inbihits proliferation of mice sarcoma S- ₁₈₀ cell.