分次大剂量L-精氨酸腹腔内注射致大鼠急性坏死性胰腺炎模型的研究

为建立一种操作简单、病变典型且稳定性好的大鼠急性坏死性胰腺炎 (ANP)模型 ,将大鼠腹腔内分次注射大剂量L 精氨酸 ( 2× 2 .5mg/g) ,注射后 1 2、2 4、4 8、72h检测其血清淀粉酶、胰腺病理变化及胰腺组织湿 /干质量比率的变化等。结果 :分次注射大剂量L 精氨酸后 ,血清淀粉酶升高 ,胰腺变性坏死 ,胰腺组织湿 /干质量比率增高。提示 :腹腔内分次注射大剂量L 精氨酸诱发的大鼠急性坏死性胰腺炎模型操作简单、可重复性好

Improvement of Experimental Animal Model of Acute Necrotizing Pancreatitis Induced by Excessive Dose of L-Arginine

To establish a simple,typical experimental animal model of acute necrotizing pancreatitis, rats were given 2.5 mg/g body weight of L Arg intraperitoneal injection twice at an interval of 1h. Blood samples were collected for determination of serum amylase, and the pancreas were removed for the histological examination and the pancreatic wet/dry weight ratio delection. Result: The serum amylase of rats given L Arg were significantly increased.The histological examination revealed that pancreas degeneration and necrosis,and the pancreatic wet/dry weight ratio were markedly increased. It shows that the administration of L Arg overdoses seperately can induce a simple, reproductive experimental animal model of ANP.