脱细胞骨软骨支架复合自体骨髓间充质干细胞修复羊骨软骨缺损的研究

目的探讨脱细胞骨软骨支架接种自体骨髓间充质干细胞(BMSCs)修复羊骨软骨缺损效果,探索骨软骨缺损新的修复方式。方法制备直径为8mm骨软骨脱细胞支架,培养羊BMSCs,接种于骨软骨支架,制备羊负重区骨软骨缺损模型,分空白、空白支架及细胞支架复合物3组,每组4只羊,3个月后处死动物取标本行大体及组织学检测。结果修复羊负重区骨软骨缺损模型实验结果显示细胞支架复合修复组骨软骨有较好修复,空白支架组软骨下骨基本修复、软骨侧无明显修复,空白对照组未见明显修复,缺损边缘软骨退变。结论含骨软骨连接结构的脱细胞骨软骨支架接种种子细胞能较好的修复羊负重区骨软骨缺损。

Acellular osteochondral scaffold combined with autologous MSCs repair goat osteochondral defects

Objective Observe the repairing effect of acellular osteochondral scaffold,which was seeded with mesenchymal stem cells（MSCs）,and was implanted in animal osteochondral defect model and to find a new way to repair osteochondral defect.Methods Osteochondral defects was prepared in weight bearing area of goat.The goats were separated into scaffold-cell group,scaffold group and blank-control group.Osteochondral scaffold was cross-linked with porous acellular cartilage matrix,seeded with BMSCs,and implanted in animal osteochondral defect model of scaffold-cell group.Pure scaffolds were implanted into scaffold group,and no repair was done in the blank-control group.The animals were sacrificed three months after the operation and the specimens had gross and histological evaluation.The effect of cartilage formation,cell biology and subchondral bone changes were observed.Results Cell-scaffold composite group had better repair for osteochondral defect of weight-bearing area than scaffold group which only had subchondral bone repaired.In the blank group the defect was repaired by fibrous tissue with the cartilage degeneration in defects＇ edge.Conclusions Extracellular matrix osteochondral scaffold seeded with MSCs can repair osteochondral defects better in load bearing area of goat.