林下仿生态种植金线莲的质量标准研究

目的对林下仿生态种植金线莲的质量标准进行研究。方法建立林下仿生态种植金线莲的性状、显微特征、薄层鉴别及含量测定方法,并按《中国药典》2015年版有关规定对水分、灰分和酸不溶性灰分等项目进行了测定。结果林下仿生态种植金线莲在性状、显微特征和薄层色谱方面均具有专属性特征,经测定5个基地的金线莲水分平均为8.69%,总灰分平均为11.93%,酸不溶性成分平均为3.27%;含量测定结果槲皮素平均质量分数为0.021 0%,异鼠李素平均质量分数为0.024 7%,山柰素平均质量分数为0.027 3%。结论上述方法简便、专属性好,重现性好,可为林下仿生态种植金线莲质量标准的制定提供依据。

Study on quality standard of imitation ecological planting Anoectochilus roxburghii under forest covering

Objective To study the quality standard of the imitation ecological planting Anoectochilus roxburghii under forest covering. Methods The identification of medicinal properties, microscopic characteristics, thin-layer chromatography (TLC) and content determination of A. roxburghii cultivated under forest were carried out in this study, and the moisture content, ash and acid-insoluble ash were determined according to Chinese Pharmacopoeia of 2015 edition. Results A. roxburghii cultivated under forest exhibits specific properties in characteristics, microscopic features and TLC results. The average moisture content of A. roxburghii cultivated under forest from five planting bases was 8.69%, the average ash was 11.93%, and the average acid-insoluble ash was 3.27%. Content determination results of the average quality score of quercetin, isorhamnetin and kaempferide were at 0.021 0%,,0.024 7%, and 0.027 3%, respectively. Conclusion The above method is simple, specific and reproducible, which will provide the basis for the quality standard of the imitation ecological planting A. roxburghii under forest covering.