广金钱草总黄酮胶囊对照品的研究(英文)

目的:为广金钱草总黄酮胶囊制备含量测定用化学对照品。方法:采用常规柱层析结合制备型HPLC分离目标化合物,通过HPLC法测定其纯度和稳定性及其在胶囊中的含量,根据光谱数据(UV、IR、ESI-MS、1H-NMR、13C-NMR、DEPT、1H-13CCOSY、1H-1HCOSY、1D-HOHAHA、1D-NOE、HMBC)鉴定其结构。结果:分离到的化合物被鉴定为异夏佛塔苷,其纯度在99以上,常温条件下放置3个月内稳定,其溶液在常温条件下8h内稳定。异夏佛塔苷在广金钱草总黄酮胶囊中含量为3.0以上。结论:该化合物可作为广金钱草总黄酮胶囊的含量测定用对照品,并且能从广金钱草中分离得到。

Studies on reference substance of Guangjinqiancao Zonghuangtong Capsule

AIM:To prepare a compound as the chemical reference substance of Guangjinqiancao Zonghuangtong Capsule.METHODS:To apply general column chromatography combined with preparative HPLC to isolate the target compound,to use analytic HPLC to determine the purity,stability and its content in the capsule,and to employ spectroscopic analysis(UV,IR,ESI-MS,1H-NMR,(13)C-NMR,DEPT,1H-(13)CCOSY,1H-1HCOSY,1D-HOHAHA,1D-NOE,HMBC) to elucidate the structure of the isolated compound.RESULTS:The obtained compound was identified as isoschaftoside with the purity of over 99%,which was stable within 3 months at ambient temperature.As for isoschaftoside solution,it was stable within 8 h at ambient temperature.Its content in the capsule was above 3.0%.CONCLUSION:Isoschaftoside is a qualified reference substance for analytic assay of Guangjinqiancao Zonghuangtong Capsule,and can be isolated from Desmodium styracifolium(Osb.) Merr.