益黄散治疗小儿慢性腹泻的网络药理学研究

目的 采用网络药理学探讨益黄散治疗小儿慢性腹泻的分子作用机制,为临床用药提供依据.方法 通过中药系统药理学数据库与分析平台(TCMSP)挖掘益黄散药物有效成分,导入PubChem、SwissTargetPrediction数据库预测有效成分潜在靶点;在GeneCards数据库中检索小儿慢性腹泻靶点,将有效成分靶点与疾病...

Study on Yihuang Powder for the Treatment of Infantile Chronic Diarrhea Based on Network Pharmacology

Objective To study the molecular mechanism of Yihuang Powder for the treatment of infantile chronic diarrhea with network pharmacology;To provide basis for clinical medication. Methods The active components of Yihuang Powder were dug from TCMSP, and then PubChem and SwissTargetPrediction databases were imported to predict potential targets of active components;the targets of infantile chronic diarrhea were searched in GeneCards database, the intersection of active component targets and disease targets was taken, and the Chinese materia medica-active components-disease network was drawn. The protein-protein interaction network was constructed by STRING 11.0 database and Cytoscape 3.8.1 Network analyzer was used to integrate and construct the multi-level network relationship of medicine composition-target-disease. GO function enrichment and KEGG pathway enrichment analysis of intersection targets were carried out through DAVID database. Results Totally 19 active components, 541 medicine targets of Yihuang Powder, 2 574 disease targets of infantile chronic diarrhoea, and 134 intersection targets of the two were collected and screened, among which the gene targets of the PIK3 CA, STAT3, VEGFR, AKT1, and EGFR were obvious. GO function enrichment resulted in 358 biological process entries, 4 cell component entries and 42 molecular function entries;KEGG pathway was enriched with 82 signaling pathways, mainly regulating cancer pathway, proteoglycan in cancer, Ras signaling pathway, PI3 K-Akt signaling pathway, focal adhesion, MAPK signaling pathway, etc.Conclusion This study reveals the multi-channel, multi-target and multi-component characteristics of Yihuang Powder in the treatment of infantile chronic diarrhea, so as to provide a theoretical basis for follow-up research.