基于UPLC-Q-TOF-MS的葛根、粉葛大鼠血清药物化学比较研究

应用UPLC-Q-TOF-MS技术和血清药物化学方法对葛根、粉葛水提取物灌胃给药后大鼠血清中移行成分进行比较研究.灌胃给予大鼠葛根、粉葛浸膏粉末溶液后,眼眶静脉从采血,血清经过甲醇-乙腈(1∶1)蛋白沉淀法处理,采用Agilent ZORBAX RRHD SB-C18色谱柱(3 mm× 100 mm,1.8 μm),A...

Comparative study of rat serum pharmacochemistry between Puerariae Lobatae Radix and Puerariae Thomsonii Radix based on UPLC-Q-TOF-MS

UPLC-Q-TOF-MS and serum pharmacochemistry were employed to study the migrating components in rat sera after intragastric administration of the water extracts of Puerariae Lobatae Radix(PLR)and Puerariae Thomsonii Radix(PTR).After the respective intragastric administration of PLR and PTR extracts,blood samples were collected from the orbital vein.The serum samples were treated by protein precipitation method with methanol and acetonitrile at a ratio of 1∶1 and then passed through Agilent ZORBAX RRHD SB-C₁₈ column(3 mm×100 mm,1.8μm)and Agilent SB-C₁₈ pre-column(3 mm×5 mm,1.8μm)with 0.1%formic acid aqueous solution(A)-acetonitrile(B)as the mobile phase.The elution was performed at the flow rate of 0.25 mL·min^-1,the column temperature of 40℃,and the injection volume of 2μL.By comparison of the total ion chromatogram and secondary fragment ion information of PLR and PTR water extracts,PLR-and PTR-containing sera,and blank serum,we found 42 migrating components(including 17 prototype components and 25 metabolites)in the sera of rats treated with PLR and 35 migrating components(including 15 prototype components and 20 metabolites)in the sera of rats treated with PTR.Thirty-three common components were shared by the two treatments,including 13 prototype components and 20 metabolites.The differences of migrating components in the PLR-and PTR-treated rat sera provide a scientific basis for further study of the active components and quality markers of PLR and PTR.