泻白散大鼠体内入血成分研究

目的研究泻白散的体内入血成分。方法采用超高效液相色谱-四极杆飞行时间质谱技术进行分析。将SD大鼠随机分为空白组和给药组,每组10只。空白组大鼠灌胃水,给药组大鼠灌胃2 g/mL(以生药量计)泻白散溶液,给药体积均为11.3 mL/kg,每日2次,连续3 d。末次给药1.5 h后,各组大鼠腹主动脉取血,将血清处理后取上清液进样分析;采集正、负离子模式下的相关数据,利用自建二级质谱数据库并查阅相关文献对泻白散入血成分进行分析鉴定。结果共鉴定出17种泻白散入血成分,其中6种来源于君药桑白皮,7种来源于臣药地骨皮,12种来源于佐使药甘草,分别为地骨皮甲素、绿原酸、tachiogroside B、astringin、新甘草苷、甘草素、壬二酸、异甘草苷、glycyroside、芒柄花苷、癸二酸、parthenolide、刺芒柄花素、18β-甘草次酸、6-姜酚、棕榈酰胺、芥酸酰胺,这些化合物主要是黄酮类、生物碱类和有机酸类成分。结论本研究初步确定了泻白散的17种入血成分,这些成分与泻白散的作用相一致,可能是泻白散的药效物质。

Study on absorbed components of Xiebai powder in rat blood

OBJECTIVE To study the absorbed components of Xiebai powder in blood.METHODS UPLC-Q-TOF-MS/MS method was adopted.SD rats were randomly divided into blank group and administration group,with 10 rats in each group.Blank group was given water intragastrically,and administration groups were given 2 g/mL(by the amount of crude drug)Xiebai powder solution intragastrically.Administration volume was 11.3 mL/kg,twice a day for 3 days.One point five hours after last administration,blood was taken from the abdominal aorta of each rat,the serum was processed to obtain the supernatant for analysis;the relevant data in positive and negative ion mode were collected,and the absorbed components of Xiebai powder in blood were analyzed and identified by using self-built secondary mass spectrometry database and consulting the relevant literature.RESULTS Totally 17 components from Xiebai powder were identified,among which 6 components came from sovereign Moru salba,7 from minister Cortex Lycii,12 from assistant Glycyrrhiza uralensis,i.e.kukoamine A,chlorogenic acid,tachiogroside B,astringin,neoglycyrrhizin,glycyrrhizin,azelaic acid,isoglycyrrhizin,glycyroside,anthocyanin,sebacic acid,parthenolide,anthocyanin,18β-glycyrrhetinic acid,6-gingerol,palmitoamide,erucamide.These compounds were mainly flavonoids,alkaloids and organic acids.CONCLUSIONS In this study,17 absorbed components of Xiebai powder in blood are preliminarily determined,which are consistent with the effect of Xiebai powder.They may be the pharmacodynamic substances of Xiebai powder.