RP-HPLC法同时测定人血浆中多种抗癫痫药物浓度

目的建立RP-HPLC法同时测定人血浆中苯巴比妥、苯妥英钠、卡马西平浓度,为临床癫痫患者个体化治疗方案的实施提供指导。方法血清样品经乙醚提取后Agilent ZORBAX SB-C 18色谱柱(150mm×4.6mm,5μm)进行分析,流动相:甲醇-水(40∶60);流速:1.0mL·min^-1;检测波长:220nm;柱温:30℃;三种药物互为内标。结果在一定范围内(苯巴比妥2~98mg·L^-1,r=0.9990;苯妥英钠1.005~50.25mg·L^-1,r=0.9992,卡马西平0.4~30mg·L^-1,r=0.9995)各药物与内标峰面积比与浓度呈良好的线性关系。相对回收率分别为101.10%、102.21%和100.12%;低、中、高浓度质控品回收率均>90%(n=5),日内、日间变异均<10%(n=5)。结论本方法操作简单、成本低、精密度及回收率均满足生物样本分析要求,结果稳定可靠,适合在基层医院开展。

Simultaneous Determination of Concentrations of Various Anti-epileptic Drugs in Human Plasma by RP-HPLC

OBJECTIVE To establish an RP-HPLC method for simultaneous determination of various anti-epileptic drugs in human plasma and provide guidance for individualized treatment regimens for epilepsy patients.METHODS Serum samples were extracted by diethyl ether and measured by RP-HPLC.The analysis was performed on an Agilent ZORBAX SB-C 18 column(150mm×4.6mm,5μm)with the mobile phase consisting of methanol and water(40∶60)at a flow rate of 1.0mL·min^-1.The temperature was 30℃,detection wavelength was 220nm,and the three drugs were used as the internal standard each other.RESULTS There was a good linear relationship in the concentration of(phenobarbital 2-98mg·L^-1,r=0.9990;phenytoin 1.005-50.25mg·L^-1,r=0.9992,carbamazepine 0.4-30mg·L^-1,r=0.9995).The relative recovery rates were 101.10%,102.21%and 100.12%;The average recovery of low,middle and high concentrations was more than 90%(92%-109%,n=5);the RSDs of intra-day and inter-day variation were less than 10%(n=5).CONCLUSION This method is economical,user-friendly and the precision and recovery rate can satisfy the requirements of biological sample analysis.With stable and reliable results,this method can be used for therapeutic drug monitoring in grassroots hospitals.