经典名方当归补血汤物质基准量值传递分析

为阐明当归补血汤物质基准的关键质量属性,通过制备21批物质基准,分别建立2套当归补血汤物质基准指纹图谱及其含量测定的检测方法,明确其指纹图谱的峰归属,相似度范围,浸出物范围,指标性成分阿魏酸、毛蕊异黄酮葡萄糖苷和黄芪甲苷的含量以及转移率范围.结果 表明,不同批次物质基准的指标性成分含量和转移率如下:当归中指标性成分阿魏...

Quality value transmitting of substance benchmarks in Danggui Buxue Decoction

To clarify the key quality attributes of substance benchmarks in Danggui Buxue Decoction(DBD),this study prepared 21 batches of DBD substance benchmarks,and established two methods for detecting their fingerprints,followed by the identification of peak attribution and similarity range as well as the determination of extract and transfer rate ranges and contents of index components ferulic acid,calycosin-7-O-β-D-glucoside,and astragalosideⅣ.The mass fractions and transfer rates of DBD substance benchmarks from different batches were calculated as follows:ferulic acid(index component in Angelicae Sinensis Radix):0.037%-0.084%and 31.41%-98.88%;astragalosideⅣ(index component in Astragali Radix):0.021%-0.059%and 32.18%-118.57%;calycosin-7-O-β-D-glucoside:0.002%-0.023%and 11.51%-45.65%,with the extract rate being 18.4%-36.1%.The similarity of fingerprints among 21 batches of DBD substance benchmarks was all higher than 0.9.The quality control method for DBD substance benchmarks was preliminarily established based on the HPLC fingerprint analysis and index component determination,which has provided a basis for the subsequent development of DBD and the quality control of novel related preparations.