鼻息肉组织中检测CD3和CD69的表达

目的　研究鼻息肉组织中T淋巴细胞表面标记CD3和早期活化标记CD69的表达,探讨人鼻息肉组织中T淋巴细胞的浸润和活化状况。方法　采用流式细胞术检测 21例鼻息肉患者鼻息肉组织、外周血中T淋巴细胞CD3、CD69的表达,并与健康人下鼻甲黏膜及外周血进行比较。结果鼻息肉组织中有大量T淋巴细胞浸润 (39.65±2.08)% ];鼻息肉组织及患者外周血T淋巴细胞均表达CD69分子,其水平分别占T淋巴细胞总量的 (36.96±2.50)%和 (4.66±0.18)%,在用多克隆刺激剂短期(5h)刺激后,两者CD69的表达均增加分别为(59.88±2.59)%、(92.76±0.55)% ];而在健康人下鼻甲黏膜中几乎未见T淋巴细胞,健康人外周血T淋巴细胞在刺激前CD69的表达较低 (1.82±0.25)% ],但在刺激后几乎全部活化 (98.54±0 28)% ]。结论　鼻息肉组织中有大量T淋巴细胞浸润且高度表达CD69分子,提示鼻息肉组织中T淋巴细胞处于异常免疫活化状态。

Examination expression of CD3 and CD69 in nasal polyps

OBJECTIVE: To investigate the infiltration of T lymphocytes with CD3 expression as surface marker and the activation of T lymphocytes with CD69 expression as activation marker. METHODS: Nasal polyp tissue samples and peripheral blood were obtained from 21 patients. The normal inferior turbinate mucosa and peripheral blood were obtained as comparison. Flow cytometry was adopted to detect the expression of CD3 and CD69 of T lymphocytes. RESULTS: Nasal polyp tissue consisted of abundant T lymphocytes. Activation marker CD69 was expressed in T lymphocytes of nasal polyps (36.96 +/- 2.50)% and peripheral blood (4.66 +/- 0.18)% from the same patient. The expression rates of CD69 after a short-term stimulation (5 h) in response to PDB were (59.88 +/- 2.59)% and (92.76 +/- 0.55)% respectively. While T lymphocytes was rarely detected in normal inferior turbinate and the expression of CD69 was low in peripheral blood from normal human but almost all T lymphocytes were activated after stimulation. CONCLUSIONS: There were generous of T lymphocytes infiltrating in nasal polyps. The expression of CD69 in T lymphocytes was abnormally high, which indicated that T lymphocytes infiltrating in nasal polyps were in activated state immunologically.