| 新的红细胞生成刺激蛋白体内生物学活性检测(英文) |
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| 引用本文： | 苏冬梅,赵会林,夏焕章. 新的红细胞生成刺激蛋白体内生物学活性检测(英文)[J]. 中国药学, 2010, 19(1): 43 |
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| 作者姓名： | 苏冬梅 赵会林 夏焕章 |
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| 作者单位： | 沈阳药科大学生命科学与生物制药学院;沈阳三生制药有限责任公司; |
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| 摘 要： | 本文建立了一种新的红细胞生成刺激蛋白体内生物学活性测定方法。将新的红细胞生成刺激蛋白单剂量注射给小鼠后, 小鼠外周血网织红细胞含量随注射剂量增加而升高。在3.125 200 ng/只的剂量范围内, 外周血网织红细胞的含量与注射剂量呈很好地线性关系, 满足量-反应平行线法定量的要求。本文对方法的准确性、精密度、检测范围、采血时间等进行了研究。结果表明, 该方法准确、重现性好, 可用于新的红细胞生成刺激蛋白生物学活性检测。
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| 关 键 词： | 红细胞生成素 红细胞生成刺激蛋白 生物测定 网织红细胞记数 贫血 |
In vivo bioassay to determine the potency of a new erythropoiesis stimulating protein |
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| Dong-Mei Su ,Hui-Lin Zhao ,Huan-Zhang Xia .Schoole of Life Science , Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang ,China. In vivo bioassay to determine the potency of a new erythropoiesis stimulating protein[J]. Journal of Chinese Pharmaceutical Sciences, 2010, 19(1): 43 |
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| Authors: | Dong-Mei Su Hui-Lin Zhao Huan-Zhang Xia .Schoole of Life Science Biopharmaceutics Shenyang Pharmaceutical University Shenyang China |
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| Affiliation: | Dong-Mei Su 1,Hui-Lin Zhao 2,Huan-Zhang Xia 1 1.Schoole of Life Science , Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China 2.Institute of Research , Development,Shenyang Sunshine Pharmaceutical Company Limited,Shenyang 110027,China |
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| Abstract: | A simple in vivo bioassay suitable for the routine quality control testing of a new erythropoiesis stimulating protein was developed.Subcutaneous administration of the new erythropoiesis stimulating protein to Balb/c mice in a single dose resulted in a dose-dependent increase in the number of circulating reticulocytes.Within the erythropoiesis stimulating protein dose range of 3.125 to 200 ng per mouse,there is a strong linear relationship between the dose and reticulocyte counts in the treated mice.This li... |
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| Keywords: | Erythropoietin Erythropoiesis stimulating protein Bioassay Reticulocyte count Anemia |
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