| 雌激素对人脐静脉内皮细胞HSP27表达的影响 |
| |
| 引用本文： | 周琴,;张青海,;邓华菲,;谭玉林,;曾新艳,;颜复生,;罗桐秀,;易光辉. 雌激素对人脐静脉内皮细胞HSP27表达的影响[J]. 国际病理科学与临床杂志, 2014, 0(5): 520-524 |
| |
| 作者姓名： | 周琴, 张青海, 邓华菲, 谭玉林, 曾新艳, 颜复生, 罗桐秀, 易光辉 |
| |
| 作者单位： | [1]湘南学院病理生理学教研室,湖南郴州423000; [2]南华大学第一附属医院,湖南衡阳421001; [3]湘南学院第一附属医院,湖南郴州423000; [4]南华大学心血管病研究所动脉硬化学湖南省重点实验室,湖南衡阳421001 |
| |
| 基金项目： | 国家自然科学基金（81270360）; 湖南省科技厅科技计划重点项目（2014FJ2012）; 湖南省教育厅项目（11C1179）; 湖南省郴州市科技局项目（[2011]29）; 湖南省重点建设学科（2011-76） |
| |
| 摘 要： | 目的：观察人脐静脉内皮细胞（human umbilical vein endothelial cell,HUVEC）中热休克蛋白27（HSP27）表达在雌激素（estrogen,E）诱导下的改变。方法：分别用10–9 M、10–8 M、10–7 M雌二醇（estradiol,E2）以及10–6 M雌激素受体拮抗剂他莫昔芬（tamox ifen）处理HUVEC后,采用Western blot法和RT-PCR法检测HUVEC中HSP27蛋白和m RNA的表达水平。结果：与对照组相比,无论是蛋白水平还是m RNA水平,10–9 mol/L E2对HUVEC中HSP27表达没有明显影响;10–8、10–7 mol/L E2诱导HUVEC中HSP27表达逐渐增加（P〈0.05）;而HUVEC与10–6 M他莫昔芬、10–7 M雌二醇共同孵育,其HSP27水平和单纯10–7 M雌二醇处理相比明显减少（P〈0.05）。结论：外源性E2能诱导HUVEC中HSP27的表达,呈剂量依赖性。他莫昔芬能阻断E2的这种上调HSP27的作用,提示雌激素诱导内皮细胞HSP27的表达依赖ER。
|
| 关 键 词： | 雌激素 人脐静脉内皮细胞 热休克蛋白27 |
Effects of estrogen on the expression of HSP27 in human umbilical vein endothelial cells |
| |
| Affiliation: | ZHOU Qin, ZHANG Qinghai, DENG Huafei, TAN Yulin, ZENG Xinyan, YAN Fusheng, LUO Tongxiu, YI Guanghui (1. Department of Pathophysiology, Xiangnan University, Chenzhou Hunan 423000; 2. First Hospital Affiliated to University of South China, Hengyang Hunan 421001; 3. First Hospital Affiliated to Xiangnan University, Chenzhou Hunan 423000; 4. Institute of Cardiovascular Disease, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang Hunan 421001, China) |
| |
| Abstract: | Objective： To investigate the change of the expression of HSP27 in human umbilical vein endothelial cells（HUVECs） induced by estrogen. Methods： After incubated with different concentrations of estradiol（10–9 mol/L, 10–8 mol/L, 10–7 mol/L, respectively） and 10–6 mol/L tamoxifen（an antagonist of estrogen receptor）, the expression of HSP27 protein and m RNA in HUVEC were detected by Western blot and RT-PCR, respectively. Results： Compared with the control group, the expression of HSP27 m RNA and protein in HUVEC were significantly up-regulated by E2（P〈0.05）（10–8, 10–7 mol/L E2）; however, the effect that estradiol increase the expression of HSP27 were inhibited obviously by 10–6 mol/L tamoxifen（P〈0.05）. Conclusion： The expression of HSP27 in HUVEC was increased significantly by E2 in a dose-dependent manner. Tamoxifen can inhibit these effects of E2. The data of the studies suggested that the expression of HSP27 in endothelial cells that induced by estrogen is dependent on estrogen receptors. |
| |
| Keywords: | estrogen human umbilical vein endothelial cells heat shock protein 27 |
| 本文献已被 维普 等数据库收录！ |
|
