| microRNA介导的基因工程病毒减毒株研究进展 |
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| 引用本文： | 沈小明,苏欣,孙文逵,施毅. microRNA介导的基因工程病毒减毒株研究进展[J]. 国外医学:呼吸系统分册, 2014, 0(22): 1727-1731 |
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| 作者姓名： | 沈小明 苏欣 孙文逵 施毅 |
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| 作者单位： | 南京军区南京总医院呼吸与危重症医学科,210002 |
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| 基金项目： | 国家自然科学基金资助项目（81200063、81470206）;中国博士后科学基金面上项目（2014M552701）;江苏省博士后基金资助项目（1302073c） |
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| 摘 要： | 为了安全有效地进行基因治疗，需要把转染的基因限制在特定组织中表达。应用miRNA调节病毒基因表达是一个新方法。在miRNA介导的转基因表达系统中，可以将与miRNA互补的靶序列串联插入病毒基因3’非编码区域，导致在miRNA高表达细胞中抑制病毒的表达。我们总结了miRNA介导的病毒减毒株的应用。
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| 关 键 词： | 微RNA 基因工程 病毒 减毒株 |
Advances in research of microRNA-mediated gene engineering attenuated strain of virus |
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| Shen Xiaoyue,Su Xin,Sun Wenkui,Shi Yi. Advances in research of microRNA-mediated gene engineering attenuated strain of virus[J]. Section of Respiratory System Foreign Medical Sciences, 2014, 0(22): 1727-1731 |
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| Authors: | Shen Xiaoyue Su Xin Sun Wenkui Shi Yi |
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| Affiliation: | .( Department of Respiratory and Critical Care Medicine, Nanjing General Hospital of Nanjing Military Command, Nanjing 210002 ,China) |
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| Abstract: | For safe and effective gene therapy, targeted tissue-restricted transgene expression is desirable. A new system which can regulate viral gene expression by exploiting microRNA （miRNA） has recently been developed. In miRNA-mediated transgene expression systems, tandem copies of sequences perfectly complementary to the miRNA are usually incorporated into the 3＇UTR of the viral gene,leading to the suppression of viral expression in cells expressing the corresponding miRNA. The aim of this review is to highlight the use of miRNA-mediated attenuated strain of virus. |
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| Keywords: | MicroRNA Genetic engineering Virus Attenuated strain |
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