| Neuronal-like differentiation of bone marrow-derived mesenchymal stem cells induced by striatal extracts from a rat model of Parkinson’s disease |
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| 作者姓名： | Xiaoling Qin Wang Han Zhigang Yu |
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| 作者单位： | Department of Neurology, Xuzhou Central Hospital;Department of Emergency, Dongying People’s Hospital |
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| 摘 要： | A rat model of Parkinson’s disease was established by 6-hydroxydopamine injection into the medial forebrain bundle. Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from the femur and tibia, and were co-cultured with 10% and 60% lesioned or intact striatal extracts. The results showed that when exposed to lesioned striatal extracts, BMSCs developed bipolar or multi-polar morphologies, and there was an increase in the percentage of cells that expressed glial fibrillary acidic protein (GFAP), nestin and neuron-specific enolase (NSE). Moreover, the percentage of NSE-positive cells increased with increasing concentrations of lesioned striatal extracts. However, intact striatal extracts only increased the percentage of GFAP-positive cells. The findings suggest that striatal extracts from Parkinson’s disease rats induce BMSCs to differentiate into neuronal-like cells in vitro.
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| 关 键 词： | bone marrow-derived mesenchymal stem cell Parkinson’s disease striatal extract induced differentiation nerve cell glial fibrillary acidic protein nestin neuron-specific enolase neural stem cell regeneration neural regeneration |
| 收稿时间： | 2012 Aug 28 |
Neuronal-like differentiation of bone marrow-derived mesenchymal stem cells induced by striatal extracts from a rat model of Parkinson's disease |
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| Xiaoling Qin,Wang Han,Zhigang Yu.Neuronal-like differentiation of bone marrow-derived mesenchymal stem cells induced by striatal extracts from a rat model of Parkinson's disease[J].Neural Regeneration Research,2012,7(34):2673-2680. |
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| Authors: | Xiaoling Qin Wang Han Zhigang Yu |
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| Institution: | Xiaoling Qin (Department of Neurology, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu Province, China)；Wang Han (Department of Emergency, Dongying People's Hospital, Dongying 257091, Shandong Province, China)；Zhigang Yu (Department of Neurology, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu Province, China)； |
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| Abstract: | A rat model of Parkinson’s disease was established by 6-hydroxydopamine injection into the medial forebrain bundle. Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from the femur and tibia, and were co-cultured with 10% and 60% lesioned or intact striatal extracts. The results showed that when exposed to lesioned striatal extracts, BMSCs developed bipolar or multi-polar morphologies, and there was an increase in the percentage of cells that expressed glial fibrillary acidic protein (GFAP), nestin and neuron-specific enolase (NSE). Moreover, the percentage of NSE-positive cells increased with increasing concentrations of lesioned striatal extracts. However, intact striatal extracts only increased the percentage of GFAP-positive cells. The findings suggest that striatal extracts from Parkinson’s disease rats induce BMSCs to differentiate into neuronal-like cells in vitro. |
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| Keywords: | bone marrow-derived mesenchymal stem cell Parkinson’s disease striatal extract induced differentiation nerve cell glial fibrillary acidic protein nestin neuron-specific enolase neural stem cell regeneration neural regeneration |
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