| Midkine shRNA对胃癌SGC7901细胞增殖与凋亡的影响 |
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| 引用本文： | 王庆苓,黄亚红,侯亚义.Midkine shRNA对胃癌SGC7901细胞增殖与凋亡的影响[J].现代肿瘤医学,2010,18(1). |
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| 作者姓名： | 王庆苓 黄亚红 侯亚义 |
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| 作者单位： | 1. 徐州医学院病理教研室,江苏,徐州,221004
2. 南京大学医学院免疫与生殖生物学实验室和国家生物医药技术重点实验室,江苏,南京,210093 |
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| 摘 要： | 目的:研究shRNA 干扰midkine对胃肿瘤细胞SGC7901生物学特性的影响.方法:构建midkine基因的特异性小RNA干扰质粒,采用脂质体2000转染SGC7901细胞,检测细胞的增殖和克隆形成能力以及细胞凋亡情况.结果:与对照组相比,转染重质粒后1-5天, SGC7901细胞增殖明显受到抑制(P＜0.01),细胞形成克隆数明显降低(P＜0.01),并且有明显的亚二倍体峰出现(P＜0.05).结论:针对midkine基因的特异性小RNA干扰质粒在体外能有效抑制人胃癌细胞SGC7901 midkine表达,细胞增殖及克隆形成能力减弱,细胞凋亡增加,为midkine 基因靶向治疗提供一定的实验依据.
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| 关 键 词： | 胃肿瘤细胞 细胞增殖 细胞凋亡 |
The effect of midkine shRNA on proliferation and apoptosis of the gastric carcinoma cell SGC7901 |
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| WANG Qing-ling,HUANG Ya-hong,HOU Ya-yi.The effect of midkine shRNA on proliferation and apoptosis of the gastric carcinoma cell SGC7901[J].Journal of Modern Oncology,2010,18(1). |
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| Authors: | WANG Qing-ling HUANG Ya-hong HOU Ya-yi |
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| Institution: | WANG Qing-ling1,HUANG Ya-hong2,HOU Ya-yi2 1Department of Pathology,Xuzhou Medical College,Xuzhou 221004,China,2Immunology , Reproductive Biology Laboratry,Medical School of Nanjing University & State Key Laboratory of Pharmaceutical Biotechnology,Nanjing 210093,China. |
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| Abstract: | Objective:To determine the effect of knockdown of midkine expression by shRNA on chatacteristic of human gastric carcinoma cell SGC7901.Methods:Specific shRNA plasmids to midkine were constructed,then transfected into SGC7901 by lipofectamin 2000.The cell proliferation,clony formation and apoptosis were detected.Results:Compared with control,SGC7901 cells transfected the recombinant plasmid expressing midkine shRNA,from1d to 5d cell proliferation was effectively inhibited(P<0.01),cell clones decreased and c... |
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| Keywords: | midkine shRNA |
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