| 硒拮抗氟对人肝细胞DNA损伤及凋亡的影响 |
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| 引用本文： | 夏涛,杨克敌,褚启龙,张明,刘方,陈学敏. 硒拮抗氟对人肝细胞DNA损伤及凋亡的影响[J]. 中国公共卫生, 2004, 20(3): 270-271 |
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| 作者姓名： | 夏涛 杨克敌 褚启龙 张明 刘方 陈学敏 |
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| 作者单位： | 华中科技大学同济医学院环境卫生学教研室,武汉,430030 |
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| 基金项目： | 国家自然科学基金资助项目(30 371 2 50 ) |
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| 摘 要： | 目的 研究氟、硒对离体人肝细胞DNA损伤及凋亡的作用。方法 体外培养的人肝细胞分别接触一定剂量的氟和 /或硒 12h后 ,检测肝细胞DNA的损伤率、凋亡率和细胞周期构成比。结果 氟组肝细胞DNA损伤率、凋亡率和S期细胞数均明显高于对照组和氟 硒组 (P <0 0 5) ,硒组DNA损伤率和凋亡小体育分率均高于对照组 ,但无显著性差异 (P >0 0 5)。结论 一定剂量的硒可拮抗氟对离体人肝细胞DNA损伤及诱导的肝细胞凋亡作用
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| 关 键 词： | 氟 硒 肝细胞 DNA损伤 细胞凋亡 |
| 文章编号： | 1001-0580(2004)03-0270-02 |
| 修稿时间： | 2003-06-01 |
Effects of selenium against fluoride on DNA damage and apoptosis of human hepatocytes |
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| XIA Tao,YANG Ke-di,ZHU Qi-long,et al.. Effects of selenium against fluoride on DNA damage and apoptosis of human hepatocytes[J]. Chinese Journal of Public Health, 2004, 20(3): 270-271 |
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| Authors: | XIA Tao YANG Ke-di ZHU Qi-long et al. |
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| Affiliation: | XIA Tao,YANG Ke-di,ZHU Qi-long,et al.Depart ment of Environmental Health,Tongji Medical College of Huazhong University of Sc ience and Technology |
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| Abstract: | ObjectiveTo investigate the influence of selenium and fl uoride on DNA damage and apoptosis in human hepatocytes in vitro.MethodsThe DNA damage rate,cellular apoptosis,and cell c ycle in human hepatocytes were measured after hepatocytes were incubated with fl uoride and/or selenium for 12 hours in vitro. ResultsThe DNA damage rate,apoptosis,and cell number in S phase in hepatocytes in the fluoride group were significantly higher than the co ntrol group and the fluoride-exposed and selenium-treated groups (P<0.05).Th e DNA damage rate and apoptosis in hepatocytes in the selenium group were higher than control group,but there was no significant difference between selenium gr oup and control group (P>0.05). ConclusionSome dose of selenium can antagonize DNA damag e and apoptosis in hepatocytes induced by fluoride. |
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| Keywords: | fluoride selenium hepatocyte DNA damage apopto sis |
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