| 氟对雄性大鼠生精细胞Fas蛋白表达影响的研究 |
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| 引用本文： | 徐蕊,尚卫超,刘建民,程学敏,巴月,黄辉,崔留欣.氟对雄性大鼠生精细胞Fas蛋白表达影响的研究[J].卫生研究,2010,39(3). |
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| 作者姓名： | 徐蕊 尚卫超 刘建民 程学敏 巴月 黄辉 崔留欣 |
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| 作者单位： | 1. 郑州大学公共卫生学院环境卫生学教研室,郑州,450001
2. 河南省灵宝市卫生防疫站 |
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| 基金项目： | 2005年河南省卫生厅攻关项目 |
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| 摘 要： | 目的探讨氟对大鼠生精细胞Fas蛋白表达影响。方法将30只4～5周龄雄性Wistar大鼠随机分为对照组、低剂量组、高剂量组。分别按0、10和20mg/kg染毒剂量隔日灌胃氟化钠(NaF)染毒39天,采用酸浸-氟离子选择电极法测定睾丸氟含量,用放射免疫法测定大鼠血清睾酮含量,用免疫组化(ICH)检测睾丸中Fas蛋白表达情况,同时镜检成熟精子质量。结果各染氟组大鼠睾丸氟含量均高于对照组(P0.05)且随染毒剂量的增加而逐渐升高;各染氟组大鼠血清睾酮含量低于对照组(P0.05),且随着染毒剂量的增加,睾酮含量逐渐降低;各染氟组大鼠生精细胞Fas蛋白阳性表达率高于对照组(P0.05),且随着染毒剂量的增加,Fas蛋白阳性表达率逐渐升高;各染毒组大鼠精子数量和精子活动率低于对照组(P0.05);精子畸形率高于对照组(P0.05)。结论染氟雄性大鼠血清睾酮水平降低,激活了生精细胞Fas/FasL系统,从而导致生精细胞损伤。
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| 关 键 词： | 氟 生精细胞 睾酮 Fas蛋白 |
Experimental study on Fas expression of spermatogenic cell in male rats induced by fluorine |
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| XU Rui,SHANG Weichao,LIU Jianmin,CHENG Xuemin,BA Yue,HUANG Hui,CUI Liuxin.Experimental study on Fas expression of spermatogenic cell in male rats induced by fluorine[J].Journal of Hygiene Research,2010,39(3). |
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| Authors: | XU Rui SHANG Weichao LIU Jianmin CHENG Xuemin BA Yue HUANG Hui CUI Liuxin |
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| Institution: | XU Rui,SHANG Weichao,LIU Jianmin,CHENG Xuemin,BA Yue,HUANG Hui,CUI Liuxin Department of Environment Health,College of Public Health,Zhengzhou University,Zhengzhou 450001,China |
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| Abstract: | Objective To research the effect of fluorine on the expression of Fas protein,then study the mechanism of male reproductive toxicity induced by fluoride on molecular level.Methods Thirty Wistar male rats were divided into control group,low-dose group and high-dose group.The NaF dosage for every group were 0,2 and 4g /L.The content of NaF in testis was measured by using fluorine selective electrode.Changes of testosterone and Fas protein were observed using the methods of radioimmunoassay,in situ hybridizati... |
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| Keywords: | fluorin spermatogenic cell testosterone fas |
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