Lymphocyte adhesion molecule ligands and extracellular matrix proteins in gliomas and normal brain: expression of VCAM-1 in gliomas |
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Authors: | A. Mäenpää Panu E. Kovanen Anders Paetau Juha Jääskeläinen Tuomo Timonen |
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Affiliation: | (1) Department of Pathology, P. O. Box 21 (Haartmaninkatu 3), SF-00014 University of Helsinki, Finland Tel.: 358-9-43461; Fax: 358-9-4346700; e-mail: Anna.Maenpaa@helsinki.fi, FI;(2) Department of Neurosurgery, University of Helsinki, Finland, FI |
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Abstract: | To identify antigenic differences between gliomas and normal brain, we have immunohistochemically studied the expression of lymphocyte adhesion molecules (ICAM-1, ICAM-2, ICAM-3, VCAM-1, E-selectin and CD58), epidermal growth factor receptor (EGFR) and extracellular matrix proteins (collagen IV, fibronectin, laminin, merosin, tenascin and vitronectin) in these tissues. Gliomas expressed high levels of ICAM-1, CD58 (LFA-3), EGFR, tenascin and vitronectin, whereas only very low levels were detected in normal brain. VCAM-1 expression was detected in 15 out of 25 gliomas but not in normal brain. The presence of VCAM-1 in gliomas was verified by immunoblotting and RNase protection assay, and in glioma cell lines by Northern blotting. Expression of VCAM-1 in gliomas may partially explain lymphocytic infiltration, and anti-VCAM-1 antibodies may be of potential in antibody mixtures for targeted therapy of gliomas. Received: 9 December 1996 / Revised, accepted: 20 February 1997 |
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Keywords: | Adhesion molecules VCAM-1 Extracellular matrix proteins Gliomas |
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