不同细胞因子和蛋白与妊娠期高血压综合征相关性的临床研究

目的探讨血清肿瘤坏死因子(TNF-α)、白细胞介素12(IL-12)、白细胞介素4(IL-4)、免疫球蛋白(Ig)G、IgA、IgM,补体C3、补体C4、C反应蛋白(CRP)在不同程度妊娠高血压综合征(简称妊高征)中的变化规律及其临床应用价值。方法采用酶联免疫吸附试验(ELISA)检测114例妊高征患者(其中轻度子痫前期39例、重度子痫前期75例)和93例正常晚期妊娠妇女血清TNF-α、IL-12和IL-4水平,采用免疫比浊法检测IgG、IgA、IgM、补体C3、补体C4和CRP。结果妊高征组TNF-α、IL-12和CRP明显高于正常晚期妊娠组(P均0.001),IL-4、IgG、IgM、补体C3和补体C4明显低于正常晚期妊娠组(P均0.001)。重度子痫前期组TNF-α、IL-12和CRP明显高于轻度子痫前期组(P0.05、P0.001、P0.001),IL-4、IgG、补体C3和补体C4明显低于轻度子痫前期组(P均0.05)。妊高征组、轻度子痫前期组、重度子痫前期组、正常晚期妊娠组血清CRP与IL-12、TNF-α呈正相关(P0.05),血清TNF-α与补体C3、补体C4呈负相关(P0.05)。结论妊高征患者血清TNF-α、IL-12、IL-4、IgG、IgM、补体C3、补体C4和CRP出现不同程度的异常。IL-12、IL-4、IgG、补体C3、补体C4和CRP可反映妊高征的严重程度,可作为判断病情的指标。

Clinical research on the correlationship of different cytokines and proteins with pregnancy induced hypertension syndrome

Objective To investigate the clinical significance of serum tumor necrosis factor-alpha（ TNF-α）,interleukin 12（ IL-12）,interleukin 4（ IL-4）,immunoglobulin（ Ig） G,IgA,IgM,complement（ C） 3,C4 and C-reactive protein（ CRP） in the diagnosis of pregnancy induced hypertension syndrome and the change regularity in different degrees of pregnancy induced hypertension syndrome. Methods The serum samples were collected from 114 patients with pregnancy induced hypertension syndrome and 93 healthy pregnant women. The levels of TNF-α,IL-12 and IL-4 were determined by enzyme-linked immunosorbent assay（ ELISA）,the levels of IgG,IgA,IgM,C3,C4 and CRP were determined by immunonephelometry. Results The levels of serum TNF-α,IL-12 and CRP in pregnancy induced hypertension sydrome group were higher significantly than those in healthy pregnant group（ P〈0. 001）. The levels of serum IL-4,IgG,IgM,C3 and C4 in pregnancy induced hypertension syndrome group were lower than those in healthy pregnant group（ P〈0. 001）. The levels of serum TNF-α,IL-12 and CRP in prophase of heavy degree eclampsia were higher significantly than in prophase of light degree eclampsia（ P〈0. 05,0. 001 and 0. 001）,and the levels of serum IL-4,IgG,C3 and C4 were lower（ P〈0. 05）. There was a positive correlation of serum CRP with IL-12 and TNF-α among pregnancy induced hypertension syndrome,prophase of light degree eclampsia,prophase of heavy degree eclampsia and healthy pregnant groups（ P〈0. 05）. There was a negative correlation of serum TNF-α with C3 and C4 among pregnancy induced hepertension sydrome,prophase of light degree eclampsia,prophase of heavy degree eclampsia and healthy pregnant groups,respectively（ P〈0. 05）. Conclusions The levels of serum TNF-α,IL-12,IL-4,IgG,IgM,C3,C4 and CRP are different-degree abnormal in pregnancy induced hypertension syndrome group,and the levels of serum IL-12,IL-4,IgG,C3,C4 and CRP reflect the severity degree of pregnancy induced hypertension syndrome,and can