IFNγ对U937细胞C1抑制物合成调控作用的研究

为研究ＩＦＮγ对Ｃ１抑制物（Ｃ１ＩＮＨ）合成的调控作用，采用ＥＬＩＳＡ双夹心法检测了经不同剂量ＩＦＮγ刺激后Ｕ９３７细胞培养上清中Ｃ１ＩＮＨ的含量。结果表明：经ＰＭＡ预先作用７２ｈ后的Ｕ９３７细胞本身能合成微量Ｃ１ＩＮＨ，但在培养第２天后才能检测到。加入ＩＦＮγ作用后，细胞Ｃ１ＩＮＨ合成增加，培养第１天即可测得。说明ＩＦＮγ对Ｕ９３７细胞Ｃ１ＩＮＨ的合成有上调作用并呈时间和剂量依赖性，当其剂量达到１０００～１００００ｕ／ｍｌ时，与对照相比Ｃ１ＩＮＨ合成量有极显著性增加（Ｐ＜０．００１）。

STUDY ON THE IMMUNOLOGICAL RELATIVE OF NUCLEAR MATRIX TO CHROMATIN FROM HUMAN ESOPHAGEAL CANCER CELLS

In order to investigate the relationship between esophageal cancer cell and its subnuclear structure,the polyclonal antiserum against the NMPs(nuclear matrix proteins) of human esophageal cancer cells gained by immunizing the rabbit with the NMPs of esophagear cancer cells was primarily purified by immuno absorbed with normal esophageal tissues.The antiserum was used to detect the NMPs,chromatin,binded proteins of active genes and high salt resistant parts of chromatin in normal and esophageal cancer cells by Western Blot.The results showed that the 120KD proteins in nuclear matrix of esophageal cancer cells did not exist in normal cells.And the chromatin,high salt resistant parts in esophageal cancer cells also showed strong immunological cross reaction.This study suggests that NMPs and chromatin are probably involved in the carcinogenesis and development of human esophageal cancer.