谷氨酸对体外培养大鼠神经细胞毒性机理的研究

为进一步探讨谷氨酸（Ｇｌｕ）的神经毒性机理，采用体外培养大鼠大脑皮质神经细胞１２～１４天，再分别加入Ｇｌｕ（Ｇｌｕ组），Ｇｌｕ＋尼莫地平（拮抗剂Ｉ组），Ｇｌｕ＋ＭＫ－８０１（拮抗剂Ⅱ组），检测各组神经细胞胞浆总钙（ＴＣａ）及游离钙（Ｃａ＾２＋ｉ）。结果显示：Ｇｌｕ组ＴＣａ和Ｃａ＾２＋ｉ明显高于正常对组（Ｐ〈０．０５），拮抗Ⅰ、Ⅱ组ＴＣａ、Ｃａ＾２＋ｉ均高于正常对照组（Ｐ〈０．０５）而低于Ｇｌｕ组＿

Study on the mechanism of glutamate mediated neurotoxicity by cortical neuron culture technique in vitro]

This study was intended to further explain the mechanism of glutamate (Glu) mediated neurotoxicity. The concentrations of TCa and Ca2+ i were measured in 32 cortical neuron cultures, which were divided into four groups: normal control group (n = 8); 0.5 mmol/L Glu group (Glu group n = 8); 0.5 mmol/L Glu + 100 mmol/L Nimodipine group (antagonist I, n = 8); 0.5 mmol/L Glu + 12 mumol/L MK-801 group (antagonist II, n = 8). The results showed that TCa and Ca2+ i concentrations in Glu group were significantly higher than those in normal control group (P < 0.05); TCa and Ca2+i in both antagonist I and II groups were evidently lower than those in Glu group (P < 0.05); No difference was found between antagonist I and II groups. The results suggest that Glu neurotoxicity is due to the intracellular calcium overload, which may be from the pathway of voltage-dependent calcium channels (VDCCs) and NMDA receptor-operated channels (NROCs).