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聚焦导析法分离人血清载脂蛋白CⅢ亚型
引用本文:方定志 龚仁蓉. 聚焦导析法分离人血清载脂蛋白CⅢ亚型[J]. 华西医科大学学报, 1999, 30(1): 111-113
作者姓名:方定志 龚仁蓉
摘    要:用密度梯度超速离心法,从混合人血清中分离极低密脂蛋白,经脱脂后再进行聚焦层析分离其中的载脂蛋白。

关 键 词:聚焦层析法 载脂蛋白C 血清诊断 亚型

Isolation of isoforms of apolipoprotein CIII from human serum by chromatofocusing]
D Fang,R Gong,K O. Isolation of isoforms of apolipoprotein CIII from human serum by chromatofocusing][J]. Journal of West China University of Medical Sciences, 1999, 30(1): 111-113
Authors:D Fang  R Gong  K O
Affiliation:Department of Biochemistry, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Abstract:This study aimed to isolate isoforms of apolipoprotein (apo) C III from human serum. 24-hour fasting serum from normal and hyperlipidemic subjects was pooled and subjected to ultracentrifugation at plasma density for 20 hours. Very low density lipoprotein (VLDL) was collected at density of d < 1.006 g/ml, and it was delipidated by ethanol and ether. The delipidated apo-VLDL was dissolved in a solution containing 7.2 mol/L urea and 20 mmol/L dithiothreitol. The insoluble apo B was removed by centrifugation. The soluble apo-VLDL was applied to PBE94 column, and eluted with elution buffer containing polybuffer 74 and 8 mol/L urea (1:8, pH4.0). After pooled, the eluted peaks of apolipoproteins were applied to column chromatography of hydroxylapatite to remove the polybuffer. The purified isoforms of apoC III and the purified apo C I, C II and E, were characterized by isoelectrofocusing and west blot. The results showed that the purified apoC III1, C III2, and C II were pure.
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