高效毛细管电泳法快速测定内江猪血清头孢唑啉钠

报道用高效毛细管电泳技术快速测定内江猪血清头孢唑啉钠（ｃｅｆａｚｏｌｉｎｓｏｄｉｕｍ，ＣＥＺ）的方法，血清用乙腈去除蛋白质，经离心后上清直接进样，毛细管血径７５μｍ，在５０ｍｍｏｌ／Ｌ碳酸钠／碳酸氢钠ｐＨ１０）中，电压２０ＫＶ分离５分钟，２５４ｎｍ紫外检测，此方法操作简单，测定迅速，线性范围为５－３２０μｇ／ｍｌ，日内相对标准偏差为１．７％，日间相对标准偏差为８．０％，平均加样回收率为１０７．９％

Rapid determination of cefazolin in porcine serum by high performance capillary electrophoresis]

A rapid high performance capillary electrophoretic method was established to assay cefazolin in porcine blood serum. Porcine serum was deproteined by 60% acetonitrile and superlants were injected with 10 s pressure load. The final assay condition was: 40 cm x 75 microns silica capillary, 50 mmol/L bicarbonate buffer at pH10, 20 kV for 5 min, 254 nm UV detection. The assay was linear within the range of 5-320 micrograms/ml, the intra-day and inter-day relative standard deviations ranged from 1.7% to 8.0%. Analytical recovery of cefazolin was 107.9%, the limit of detection was 1.5 micrograms/ml, the limit of quantitation was 3.6 micrograms/ml. To investigate the pharmacokinetic profile of cefazolin in pig, six adult pigs were injected with 16 mg/kg cefazolin and all data was analyzed by pharmacokinetic software 3P87. Cefazolin metabolism in adult pigs appeared to be a typical two-compartment model with a clearance of 8.43 ml/(kg.min).