免疫刺激对大鼠海马CA2、3区nNOS和c-Fos表达的影响

目的通过观察细菌脂多糖(LPS)对海马CA2、3区nNOS和c-Fos表达的影响,探讨海马CA2、3区的免疫调节作用及其与NO和c-Fos蛋白的相关性。方法实验组SD大鼠腹腔注射LPS,对照组注射生理盐水,2.5 h后,采用RT-PCR方法检测大鼠海马CA2、3区nNOS mRNA、原位杂交技术检测该区c-Fos mR-NA,免疫组化方法分别检测该区nNOS和c-Fos的表达变化。结果对照组和腹腔注射LPS组大鼠海马CA2、3区的nNOS,c-Fos及其mRNA的OD值如下:nNOS mRNA(0.283±0.09,0.476±0.03),nNOS(0.653±0.97,1.155±0.12),c-Fos mRNA(1.031±0.99,1.326±0.91),c-Fos(0.426±0.16,0.830±0.14);LPS使大鼠海马CA2、3区nNOS和c-Fos mRNA及表达产物含量均上调。结论海马CA2、3区在机体的免疫应激反应中起重要调节作用,NO和c-Fos蛋白可能是该调节过程中的重要信使分子。

Effects of Immune Stimulation on the Expression of nNOS and c-Fos in Hippocampal CA2 and CA3 Regions of Rat

Objective To observe the effects of lipopolysacharide(LPS) on the expression of nNOS and c-Fos in hippocamapl CA2~3 regions and investigate the roles of NO and c-Fos in the neuroimmunomodulation of hippocampal CA2~3 regions.Methods SD rats of experiment group were intraperitoneally injected by LPS,control rats were treated by normal saline. 2.5h after treatment,RT-PCR was used to detect the expression of nNOS mRNA,in situ hybridization to detect c-FosmRNA,and immunohistochemistry to detect nNOS and c-Fos.Results The optic density(OD) values of nNOS,cFos and their mRNAs in rat hippocampal CA2~3 regions were showed as follows: nNOS mRNA(0.283±0.09,0.476±0.03),nNOS(0.653±0.97,1.155±0.12),c-Fos mRNA(1.031±0.99,1.326±0.91),c-Fos(0.426±0.16,0.830±0.14) in control and LPS groups respectively.The present results indicate that LPS upregulates the expressions of nNOS,c-Fos and their mRNA in rat hippocampal CA2 and CA3 areas.Conclusion Rats hippocampal CA2 and CA3 regions play an important role in the immunomodulating process in which NO and c-Fos are closely involved.