一氧化氮合酶和IL10基因表达对肝细胞再生的影响

目的：探讨一氧化氮合酶（ｅＮＯＳ）在白介素１０（ＩＬ１０）和肝再生信息传导中的作用。方法：以７０％肝切除制备肝再生模型，注射５０％ＣＣｌ４和脾切除引起肝损伤和机体免疫功能低下，４８ｈ后采用逆转录ＰＣＲ（ＲＴＰＣＲ）和点杂交（ｄｏｔｂｌｏｔ）方法检测ｅＮＯＳ和ＩＬ１０ｍＲＮＡ基因表达，免疫组化ＬＳＡＢ法检测ＩＬ１０蛋白在肝内的分布，流式细胞仪（ＦＣＭ）检测再生肝ＤＮＡ含量，最后采用统计学作再生肝组织ＤＮＡ含量与ＩＬ１０和ｅＮＯＳ变化的相关分析，以此判断ＩＬ１０和ｅＮＯＳ的变化对再生肝的影响。结果：ｅＮＯＳ和ＩＬ１０对肝再生具有明显的调控作用，无论在生理或在病理状态下都是如此，并且二者呈现一种完全相反的调控方式，同时受到脾脏或全身免疫系统的影响。结论：ｅＮＯＳ的表达同肝细胞ＤＮＡ的合成呈正相关，而ＩＬ１０的表达与其呈负相关。

Effects of eNOS and IL 10 gene in signal transduction for liver regeneration

Objective:To detect the effects of eNOS and IL 10 on liver regeneration under physiological and pathological condition.Methods:The rat model of liver regeneration was made by partial(70%) hepatectomy method;some rats were injected with CCl 4 and performed splenectomy;the hepatocytes DNA replication were tested by flow cytometry(FCM) method and the gene expression of endothelial cells nitric oxide synthase(eNOS) in liver cells were analysed using RT PCR.The IL 10 gene expression in liver cells was showed by dot blot hybridization method;the synthesize and distribution of IL 10 protein in liver tissue were examined by immune histochemical and morphometric analysis method.Results:eNOS and IL 10 have important immunomodulatory role on liver regeneration both on physiological or pathological conditions,and the effects of eNOS and IL 10 on liver regeneration is different,which play a opposing modulatory effect on liver regeneration,meanwhile,such effect was affected by spleen and general immune system.Conclusions:The expression of eNOS gene promote liver regeneration while the expression of IL 10 gene inhibit it.