| RP-HPLC法测定杞菊地黄片中丹皮酚的含量 |
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| 引用本文： | 曾滨阳,代春美,刘彦生.RP-HPLC法测定杞菊地黄片中丹皮酚的含量[J].武警医学院学报,2009,18(8):685-686,689. |
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| 作者姓名： | 曾滨阳 代春美 刘彦生 |
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| 作者单位： | 1. 武警辽宁总队医院药剂科,辽宁,沈阳,110034
2. 辽宁医学院,辽宁,锦州,121001 |
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| 摘 要： | 【目的】建立RP-HPLC法测定杞菊地黄片中丹皮酚含量的方法。【方法】以KromasilC18液相柱（250mm×4．6min，5μm）为固定相，甲醇-0．05磷酸溶液（70：30）为流动相，检测波长为274nm，流速为1．0ml／min。【结果】丹皮酚在50～800ng范围内线性关系良好（r=0．9999），平均加样回收率为99．55（n=2）；测得杞菊地黄片中丹皮酚的含量为1．804mg/片。【结论】RP-HPLC法专属性强，重复性好，可用于杞菊地黄片的质量控制。
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| 关 键 词： | 杞菊地黄片 丹皮酚 RP-HPLC法 含量测定 |
Determination of Paeonol in Qijudihuang tablet by RP-HPLC |
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| ZENG Bin-yang,DAI Chun-mei,LIU Yan-sheng.Determination of Paeonol in Qijudihuang tablet by RP-HPLC[J].Acta Academiae Medicinae CPAPF,2009,18(8):685-686,689. |
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| Authors: | ZENG Bin-yang DAI Chun-mei LIU Yan-sheng |
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| Institution: | (Department of Pharmacy, Liaoning Provincial Corps Hospital of Chinese People＇s Armed Police Force, Shenyang 110034, China ) |
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| Abstract: | Objective] To establish a method for the determination of Paeonol in Qijudihuang tablet by RP- HPLC. Methods] The chromatographic conditions were Kronmsil C18 （250 mm× 4.6 mm, 5 μm） as analytic column, methano-0.05% orthophosphoric Acid （70：30） as mobile phase, the flow rate being 1.0 ml/min, the detection wavelength at 274 nm. Results] The linearity of Paeonol in the range of 50 - 800 ng was good, r = 0. 9999. The average recovery was 99.5% , and the content of Paeonol in Qijudihuang tablets was 1.804mg every tablet. Conclusions] The RP- HPLC method is accurate, reliable, specific, and can be used for the quality control of Qijudihuang tablet. |
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| Keywords: | Qiju dihuang tablet Paeonol RP-HPLC |
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