| 大孔吸附树脂分离纯化龙须藤总黄酮的工艺研究 |
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| 引用本文： | 徐伟,张淑玲,洪振丰,郑海音.大孔吸附树脂分离纯化龙须藤总黄酮的工艺研究[J].广东药学院学报,2009,25(2):144-146. |
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| 作者姓名： | 徐伟 张淑玲 洪振丰 郑海音 |
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| 作者单位： | 福建中医学院,药学系,福建,福州,350108;福建中医学院,中西医结合系,福建,福州,350108 |
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| 基金项目： | 福建省科技厅重点项目 |
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| 摘 要： | 目的研究AB-8大孔树脂分离纯化龙须藤黄酮的工艺条件。方法通过静态吸附-解吸附实验筛选最佳大孔树脂，并以总黄酮含量为考核指标，采用L9（3^4）正交试验法对影响AB-8大孔树脂分离纯化龙须藤黄酮的因素进行考察，确定最佳工艺条件。结果最佳工艺条件为：采用AB-8型大孔树脂，取龙须藤黄酮提取液（含生药0．5g／mL）上样，树脂用量为4倍生药量，洗脱剂采用体积分数50％乙醇，用量为16倍生药量。结论该工艺条件科学合理，可有效地用于龙须藤黄酮的分离富集。
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| 关 键 词： | 龙须藤 大孔树脂 正交设计法 总黄酮 |
Separation and purification for the total flavonoids from Bauhinia championii Benth with AB-8 macroporous adsorption resin |
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| XU Wei,ZHANG Shuling,HONG Zhenfeng,ZHENG Haiyin.Separation and purification for the total flavonoids from Bauhinia championii Benth with AB-8 macroporous adsorption resin[J].Academic Journal of Guangdong College of Pharmacy,2009,25(2):144-146. |
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| Authors: | XU Wei ZHANG Shuling HONG Zhenfeng ZHENG Haiyin |
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| Affiliation: | XU Wei1,ZHANG Shu-ling1,HONG Zhen-feng2,ZHENG Hai-yin2(1.Department of Pharmacy,2.Department of Integrated Medicine,Fujian College of TCM,Fuzhou,Fujian 350108,China) |
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| Abstract: | Objective To optimize the separation and purification procedure of total flavonoids from Bauhinia championii Benth by AB-8 macroporus resin.Methods The yield of total flavonoids was used as a maker.The static and dynamic adsorption/desorption methods and orthogonal design L9(34)were used to evaluate factors such as concentration of alcohol,ratio of alcohol to raw material and ratio of resin to raw material.The content of total flavonoids was determined by UV-Vis spectrophotometer.Results The optimum conditi... |
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| Keywords: | Bauhinia championii Benth macroporus resin orthogonal test total flavonoids |
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