Cyclic bisbibenzyls induce growth arrest and apoptosis of human prostate cancer PC3 cells |
| |
| Authors: | Ai-hui XU Zhi-min HU Jian-bo QU Shang-ming LIU Ali Kazim Abbas SYED Hui-qing YUAN Hong-xiang LOU |
| |
| Institution: | [1]Department of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Ji-nan 250012, China; [2]Department of Natural Product Chemistry, School of Pharmaceutical Sciences, Shandong University, Ji-nan 250012, China; [3]Department of Histology and Embryology, School of Medicine, Shandong University, Ji-nan 250012, China |
| |
| Abstract: | Aim:To investigate the cytotoxic effects of four cyclic bisbibenzyls, Riccardin C (Ric), Pakyonol (Pak), Marchantin M (Mar), and Plagiochin E (Pla) against chemoresistant prostate cancer PC3 cells.Methods:Cell growth was assayed by MTT method, and apoptotic related protein Bcl-2 and Bax, poly(ADP-ribose) polymerase (PARP) were examined by Western blotting. Cell cycle and apoptosis of PC3 cells were evaluated with flow cytometry and morphologic examinations.Results:The four compounds inhibited proliferation and elicited cell death in a dose- and time-dependent manner with IC50 values of 3.22 μmol/L for Ric, 7.98 μmol/L for Pak, 5.45 μmol/L for Mar, and 5.99 μmol/L for Pla, respectively. Furthermore, exposed to these chemicals caused a decrease in the antiapoptotic protein Bcl-2 and an increase in proapoptotic Bax expression. PARP cleavage and caspase-3 activity were also observed.Conclusion:The results suggest that cyclic bisbibenzyls could be used for the development of novel therapeutic chemicals against prostate cancer. |
| |
| Keywords: | cyclic bisbibenzyls liverworts apoptosis prostate carcinoma PC3 cells Bcl-2 poly(ADP-ribose) polymerase |
| 本文献已被 维普 等数据库收录！ |
|
