Efficient in vivo gene transfection by stable DNA/PEI complexes coated by hyaluronic acid |
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Authors: | Ito Tomoko Iida-Tanaka Naoko Koyama Yoshiyuki |
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Institution: | Department of Textile Science, Otsuma Women's University Graduate School, Tokyo, Japan. |
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Abstract: | Plasmid DNA was mixed with polyethyleneimine (PEI) and hyaluronic acid (HA) to afford ternary complexes with negative surface charge regardless of the mixing order. They showed reduced non-specific interactions with blood components. When DNA and PEI were mixed at a high concentration such as that used in in vivo experiments, they soon aggregated, and large particles were formed. On the other hand, pre-addition of HA to DNA prior to PEI effectively diminished the aggregation, and 10% (in volume) of the complexes remained as small particles with a diameter below 80 nm. Those negatively charged small ternary complexes induced a much stronger extra-gene expression in tumor than binary DNA/PEI complex after intratumoral or intravenous injection into the mice bearing B16 cells. |
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