| Protection of rat islet viability following heme oxygenase-1 gene transfection via adenoviral vector in vitro |
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| 作者姓名： | Xiaobo Chen Yongxiang Li Weiping Dong Yang Jiao Jianming Tan |
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| 作者单位： | [1]Department of Renal Transplantation and Urology, the First Affiliated Hospital, Shanghai Jiao Tong University, Shanghai 200080, China [2]Diabetes Research Laboratory, the First Affiliated Hospital, Shanghai Jiao Tong University, Shanghai 200080, China |
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| 摘 要： | Objective： To investigate the effect of Heme oxygenase-1 （HO-1） gene transfection on the viability of cultured rat islets, and to explore the potential value of HO-1 gene in islet transplantation. Methods：Recombinant adenovirus vector containing human HO-1 gene（Ad-HO-1 ） or enhanced green fluorescent protein gene（Ad-EGFP） was generated by using AdEasy system respectively. The rat islets were transfected with Ad-HO-1, Ad-EGFP or blank vector and then cultured for 7 days. Transfection was confirmed by expression of EGFP and human HO-1 protein detected by fluorescence photographs and western blot, respectively. The insulin release upon different concentration of glucose stimulation was detected using insulin radioimmunoassay kit, and stimulation index（SI） was calculated. Glucose-stimulated insulin release was used ＇to assess islet viability. Results：Adenovirus vector successfully transferred HO-1 gene to rat islet cells in vitro, and the insulin release upon high level of glucose stimulation and stimulation index （SI） of Ad-HO-1-infected islets were significantly higher than those of Ad-EGFP-infected islets and control islets （P 〈 0.05）. Conclusion： Adenovirus-mediated HO-1 gene transfection is a feasible strategy to confer cytoprotection and therefore protect the viability of cultured rat islets.
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| 关 键 词： | 保护作用 亚铁血红素 酶活性 基因转染 |
| 收稿时间： | 2006-09-18 |
Protection of rat islet viability following heme oxygenase-1 gene transfection via adenoviral vector in vitro |
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| Xiaobo Chen,Yongxiang Li,Weiping Dong,Yang Jiao,Jianming Tan.Protection of rat islet viability following heme oxygenase-1 gene transfection via adenoviral vector in vitro[J].Acta Universitatis Medicinalis Nanjing,2007,21(2):89-93. |
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| Authors: | Xiaobo Chen Yongxiang Li Weiping Dong Yang Jiao and Jianming Tan |
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| Abstract: | Objective: To investigate the effect of Heme oxygenase-1 (HO-1) gene transfection on the viability of cultured rat islets, and to explore the potential value of HO-1 gene in islet transplantation. Methods:Recombinant adenovirus vector containing human HO-1 gene(Ad-HO-1 ) or enhanced green fluorescent protein gene(Ad-EGFP) was generated by using AdEasy system respectively.The rat islets were transfected with Ad-HO-1, Ad-EGFP or blank vector and then cultured for 7 days. Transfection was confirmed by expression of EGFP and human HO-1 protein detected by fluorescence photographs and western blot, respectively. The insulin release upon different concentration of glucose stimulation was detected using insulin radioimmunoassay kit, and stimulation index (SI) was calculated. Glucose-stimulated insulin release was usedto assess islet viability. Results:Adenovirus vector successfully transferred HO-1 gene to rat islet cells in vitro, and the insulin release upon high level of glucose stimulation and stimulation index(SI) of Ad-HO-1-infected islets were significantly higher than those of Ad-EGFP-infected islets and control islets(P ＜ 0.05).Conclusion: Adenovirus-mediated HO-1 gene transfection is a feasible strategy to confer cytoprotection and therefore protect the viability of cultured rat islets. |
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| Keywords: | adenovirus vectors heme oxygenase-1 pancreatic islet gene transfection in vitro adenoviral vector gene transfection heme viability islet cells feasible strategy cytoprotection control high level Adenovirus transferred Results assess index release different concentration |
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