铁调素在小鼠脑内的表达及其对膜铁转运蛋白1和二价金属离子转运体1表达的影响

目的 探讨铁调素(hepcidin)在小鼠脑内的表达及其对膜铁转运蛋白1(ferroportir 1)和二价金属离子转运体1(DMT1)表达的调节作用.方法 应用RT-PCR技术检测铁调素在正常小鼠各脑区的表达分布,并观察了脑室内注射铁调素对DMT1、膜铁转运蛋白1表达的影响 结果 铁调素在小鼠脑内有广泛表达,且不同脑区表达程度不同,脉络丛部分表达较高.结论 侧脑室内注射铁调素后,能够显著影响DMT1、膜铁转运蛋白1表达,且具有明显的区域特异性.

THE EXPRESSION AND EFFECTS OF HEPCIDIN IN THE MOUSE BRAIN AND ITS MODULATING EFFECTS ON FERROPORTIN 1 AND DIVALENT METAL TRANSPORTER

Objective To investigate the distribution and role of hepcidin on ferroportin1 and divalent metal transporter 1(DMT1) expression in the mouse brain. Methods The expression of hepcidin in the mouse brain was detected by RT-PCR.The role of hepcidin on ferroportin1 and DMT1 expression was studied with cerebral ventricle injection and immunohistochemistry methods. Results There was hepcidin mRNA expression in the brain,and the level of hepcidin mRNA varied in different brain areas.Abundant expression of hepcidin existed in the choroid plexus.Conclusions The positive staining of DMT1,ferroportin1 was changed remarkably after the injection of hepcidin into cerebral ventricle of the brain;the change of DMT1 and ferroportin1 staining was regionally distinct.