| 蕲蛇药材高效毛细管电泳指纹图谱的研究 |
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| 引用本文： | 王成芳,包永睿,孟宪生,李峰.蕲蛇药材高效毛细管电泳指纹图谱的研究[J].辽宁中医杂志,2010(5):893-894. |
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| 作者姓名： | 王成芳 包永睿 孟宪生 李峰 |
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| 作者单位： | 辽宁中医药大学药学院; |
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| 基金项目： | 国家自然科学基金资助项目(30973881);;辽宁省教育厅资助项目(20060547);;沈阳市科技局资助(1081238-1-00) |
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| 摘 要： | 目的:建立蕲蛇药材高效毛细管电泳(HPCE)指纹图谱。方法:以20mmol/L硼砂-0.2mol/L硼酸(pH8.4)作为背景电解质缓冲液,柱温25℃,运行电压19kV,245nm波长处检测,25min内完全分离。结果:建立的指纹图谱中共有7个共有峰,且方法学考察达到标准。结论:该方法具有良好的精密度和分离效果,可作为蕲蛇药材的质量控制方法。
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| 关 键 词： | 蕲蛇 高效毛细管电泳 指纹图谱 |
Study on HPCE Fingerprint of Agkistrodon |
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| WANG Cheng-fang,BAO Yong-rui,MENG Xian-sheng,LI Feng.Study on HPCE Fingerprint of Agkistrodon[J].Liaoning Journal of Traditional Chinese Medicine,2010(5):893-894. |
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| Authors: | WANG Cheng-fang BAO Yong-rui MENG Xian-sheng LI Feng |
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| Institution: | College of Pharmacy Department/a>;Liaoning University of Traditional Chinese Medicine/a>;Dalian 116600/a>;Liaoning/a>;China |
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| Abstract: | Objective：To establish the high performance capillary electrophoresis（HPCE）fingerprint of agkistrodon.Methods：It was separated in 25min with 20mmol/L borate- 0.2mol/L boric acid（pH8.4）as HPCE buffer,the run voltage was 19 KV,temperature 25℃ and the DAD detection was set at 245nm.Results：Fingerprint consisted of 7 common peaks.The validation of methods was satisfied with the standards.Conclusion：This method can be used for the quality control of Agkistrodon. |
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| Keywords: | agkistrodon HPCE fingerprint |
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