鼠痘病毒潜伏感染的研究

对经实验前后的普通级和清洁级Ｋ Ｍ,ＢＡＬＢ／ｃ,Ｃ５７ＢＬ小鼠,分别用ＩＦＡ,ＥＬＩＳＡ、ＰＡＰ和ＡＢＣ法方法检测小鼠血清中的ＬＣＭＶ、ＥＨＦＶ、ＭＰＶ、ＭＨＶ,仙台病毒抗体和抗原,电镜检查组织中的病毒颗粒。结果显示：未经实验及经实验１５ｄ和３０ｄ后的抗体和抗原。电镜检查组织中的病毒颗粒。经实验６０天后检测普通级ＫＭ和ＢＡＬＢ／ｃ小鼠鼠痘各品系小鼠血清病毒抗体均为阴性,经实验６０于后检测普通级Ｋ

Study on Latent Infection of Mousepox

The conventional and clean KM, BALB/c and C57BL/6J mice were detected for LCM( lymphocytic choriomeningitis virus), EHFV( epidemic hemorrhagic fever virus),Mouse pox, MPV( mouse hepatitis virus), Sendai virus infetion by using IFA, ELISA, ABC( avidin biotin peroxidse complex), and electron microscope before and 15,30, 60 days after undergoing animal experiments . The results revealed that 60 days after the animal experiments, ectromelia virus was found in the liver , mesenteric lymphaden, Payer's patches, and spleen of seronegative(ELISA,IFA ) KM and BALB/c mice ,while in the other groups no ectromelia virus infection were found . The findings suggest that the mice with latent infection of ectromelia virus,despite of sero negative ,would give rise to an outbreak when they were under a long stress of animal experiment, and the viral antigen detection of Mousepox should be used in routine microbiological monitoring.