| RP-HPLC测定四物汤中芍药苷的含量(英文) |
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| 引用本文： | 王玉华,魏英勤,王伟,袁久荣.RP-HPLC测定四物汤中芍药苷的含量(英文)[J].中成药,2005,27(8):910-913. |
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| 作者姓名： | 王玉华 魏英勤 王伟 袁久荣 |
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| 作者单位： | 1. 山东中医药大学天然药物省重点实验室,山东,济南,250014
2. 内蒙古药品检验所,内蒙古,呼和浩特市,010020 |
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| 摘 要： | 目的:建立一种新的测定芍药苷的反相高效液相色谱流动相系统,并令人满意地测定四物汤中芍药苷的含量.方法:色谱条件:Alltima C18柱(250 mm×4.6 mm,5 μm);紫外检测器,检测波长230 nm;乙腈-0.02 mol·L-1磷酸(18:82)为流动相.结果:芍药苷在0.48μg～4.80μg范围内与峰面积积分值呈良好的线性关系,r=0.999 4.平均加样回收率为98.8%,RSD为1.32%(n=5).结论:方法准确,精密,重现性好,结果令人满意.
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| 关 键 词： | 芍药苷 白芍 四物汤 |
| 文章编号： | 1001-1528（2005）08-0910-03 |
| 收稿时间： | 2004-09-31 |
| 修稿时间： | 2004-09-31 |
Determination of paeoniflorin in Siwu Decoction by RP-HPLC |
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| WANG Yu-hua WEI Ying-qin,WANG Wei,YUAN Jiu-rong.Determination of paeoniflorin in Siwu Decoction by RP-HPLC[J].Chinese Traditional Patent Medicine,2005,27(8):910-913. |
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| Authors: | WANG Yu-hua WEI Ying-qin WANG Wei YUAN Jiu-rong |
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| Institution: | WANG Yu-hua1 WEI Ying-qin1,WANG Wei2,YUAN Jiu-rong1 |
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| Abstract: | AIM: A new RP-HPLC method had been developed and was suitable for the analysis of paeoniflorin in Siwu Decoction (Radix Paeoniae Alba;Radix Rehmaniae Preparata, Radix Angelicae Sinensis;Rhizoma Chuanxion) (SWD). METHODS: Experiment was carried out on Alltima C18 Column and UV detector (230 nm). Acewas a good linear relationship between the concentration of paeoniflorin and area integral value in the range of 0.48-4.80 μg,r =0.999 4. The everage recovery was 98.8% with RSD 1.32% (n =5). CONCLUSION: The method is accurate, precise and reproducible for analysis of paeoniflorin in SWD. The result is satisfactory. |
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| Keywords: | RP-HPLC RP-HPLC paeoniflorin Radix Paeoniae Alba Siwu Decoction (SWD) |
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