| 用小鼠骨髓内皮细胞条件培养液代替造血刺激因子培养CFU— … |
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| 引用本文： | 程腊梅,王绮如.用小鼠骨髓内皮细胞条件培养液代替造血刺激因子培养CFU— …[J].湖南医科大学学报,2000,25(6):539-541. |
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| 作者姓名： | 程腊梅 王绮如 |
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| 作者单位： | 湖南医科大学血液生理研究室,长沙 |
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| 摘 要： | 利用大于１０ｋＤ组分的小鼠骨髓内此细胞条件培养液（ｍＢＭＥＣ－ＣＭ）作刺激物体外琼脂培养ＣＦＵ－ＧＭ，与ｍＧＭ－ＣＳＦ合用培养ＨＰＰ－ＣＦＣ获得成功。就培养ＣＦＵ－ＧＭ和ＨＰＰ－ＣＦＣ而言，用细胞因子作刺激物与并用大于１０ｋＤ组分的ｍＢＭＥＣ－ＣＭ作刺激物相比较，以后者的ＣＦＵ－ＧＭ及ＨＰＰ－ＣＦＣ产率最高。
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| 关 键 词： | 骨髓内皮细胞 CFU－GM HPP－CFC 造血细胞 |
Substitution of BMEC-CM for hematopoietic stimulators in the culture of CFU-GM and HPP-CFC] |
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| L M Cheng,Q R Wang.Substitution of BMEC-CM for hematopoietic stimulators in the culture of CFU-GM and HPP-CFC][J].Bulletin of Hunan Medical University,2000,25(6):539-541. |
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| Authors: | L M Cheng Q R Wang |
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| Institution: | Research Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078. |
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| Abstract: | The effects of > 10 kD component of serum-free conditioned medium from murine bone marrow-derived endothelial cell line on the formation of CFU-GM and HPP-CFC were investigated. We found that > 10 kD component alone could stimulate CFU-GM colony formation in dose-dependent. Above- 10 kD component supplemented with GM-CSF not only resulted in significant increase of CFU-GM as compared with > 10 kD or GM-CSF alone, but also could stimulate HPP-CFC formation. The effect of > 10 kD component on the formation of HPP-CFC was also in dose-dependent. The component above- 10 kD combined with IL-3, IL-6, EPO, SCF or GM-CSF further enhanced HPP-CFC colony formation. The results suggest that > 10 kD component may be used as the substitutes of hematopoietic growth factors to stimulate the formation of CFU-GM and HPP-CFC. |
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