人参二醇组皂苷对感染性休克大鼠血液流变性及微循环的影响

目的 观察人参二醇组皂苷(PDS)对感染性休克大鼠血液流变性及微循环的影响,探讨其抗感染性休克的作用机制.方法 大鼠舌下静脉注射PDS进行预治疗,10 min后注射细菌内毒素(LPS 5 mg/kg)复制感染性休克模型.实验动物随机分为对照组(S组);内毒素休克模型组(L组);地塞米松预治疗组(LD组);PDS预治疗(22.5 mg/kg,LP_小)(45 mg/kg,LP_中)(90 mg/kg,LP_大)组.各组于休克后观察肠系膜微循环的变化,2 h和4 h腹主动脉取血,测量全血黏度.结果 模型组在休克2 h、4 h全血黏度明显高于PDS各剂量组(P＜0.01).肠系膜微循环的变化:PDS各剂量组在休克2～4 h微动脉管径、微动脉血流速度及微动脉管袢数均高于模型组(P＜0.05).结论 PDS通过降低内毒素休克大鼠全血黏度,改善微循环状态,起到抗休克的作用.

Effects of PDS on blood rheology and microcirculation of septic shock rats

Objective To observe the effects of panaxadiol saponin (PDS) on blood rheology and microcirculation of septic shock rats, and explore its anti-septic-shock mechanism. Methods Rats were given pre-treatments by sublingual venous injection of PDS and were injected lipopolysaccharide (LPS 5 mg/kg) 10 min later to generate the septic shock models. Experimental rats were randomly divided into LPS, LPS+dexamethasone(Dex), LPS+PDS (lps: 22.5, lpm: 45, lph: 90 mg/kg ) and sham group, respectively. The shocked rats of different groups were observed the changes of mesenteric microcirculation and were drawn blood from abdominal aortas 2 and 4 h later to measure the whole blood viscosity. Results After being shocked 2 and 4 h, the blood viscosity of LPS group was obviously higher than that of PDS groups (P＜0.01). The variation of mesenteric microcirculation, including the diameter, blood velocity and loop numbers of arterioles of LPS+PDS groups were all higher than those of LPS group (P＜0.05). Conclusions PDS can improve the microcirculation state of LPS-shock rats by decreasing the whole blood viscosity.