高脂饮食导致胰岛素抵抗物质基础的实验研究

目的探讨高脂饮食所致肥胖导致胰岛素抵抗的物质基础。方法将30只雄性Wistar大鼠分为正常对照组、高脂组,正常对照组喂饲普通块料,高脂组喂饱和脂肪酸提供59%热卡的高脂饲料。各组共喂饲3月后测定体重,空腹血糖,糖负荷30、60、120min血糖,空腹胰岛素,胰岛素耐量,血脂,血清游离脂肪酸谱。结果与正常对照组比较,高脂组大鼠从第4周开始体重明显升高,空腹血糖和糖负荷30、60、120min血糖,糖耐量试验中葡萄糖曲线下面积,皮下注射胰岛素后40、90、120min血糖,胰岛素耐量试验中葡萄糖曲线下面积,血清胰岛素,胰岛素抵抗指数,血清瘦素,血清胆固醇和甘油三酯含量均明显升高。高脂组大鼠血清游离脂肪酸谱中饱和脂肪酸明显升高、不饱和脂肪酸明显下降。结论肥胖导致胰岛素抵抗的物质基础与脂肪代谢异常,特别是和饱和脂肪酸的升高、不饱和脂肪酸的下降有关。

Substance basis of insulin resistance induced by high fat diet

Objective To investigate the effect of fatty on insulin resistance in high fat-fed rats. Methods Thirty male Wistar rats including the normal group and the high fat-fed group were used in study. The control rats were fed with common diet.The high fat-fed group were fed with high fat diet containing saturated free fatty acids to provide 59% caloric. All of items were detected after each group was fed for 12 weeks. Results Compared with the normal group, the body weight from week 4 to the end, fasting blood glucose and blood glucose after 30,60 and 120 min of oral glucose, the tolerance test area under the glucose curve, blood glucose after 40,90 and 120 min of insulin injection subcutaneously and the area under glucose curve in the insulin tolerance, serum insulin, insulin resistance index, serum leptin, serum cholesterol, serum triglyceride and saturated fatty acid were significantly increased in the high fat-fed group, while the unsaturated fatty acid was significantly decreased. Conclusion The substance basis of insulin resistance induced by fatty is related to abnormal lipid metabolism, higher saturated fatty acid and lower unsaturated free fatty acids.