双氯芬酸钠滴眼液在家兔房水中的吸收

 目的建立房水中双氯芬酸钠的高效液相色谱测定方法，并测定家兔用药后眼房水中药物浓度。方法采用C18色谱柱，流动相为甲醇-水-冰醋酸(80∶20∶0.5)，房水用甲醇沉淀蛋白后，上清液挥干，再用甲醇复溶后进样。结果本方法线性范围为10～500 ng·mL^-1，最低检测浓度为10 ng·mL^-1。3种浓度的平均回收率达到99.7%，日内和日间RSD均小于10%，家兔单剂量应用0.1%双氯芬钠滴眼液50μL后，房水中15 min即可检测到药物，在给药后1h达到最高浓度。结论本方法灵敏，准确，精密。适合于眼房水中双氯芬酸钠的测定。

Absorption of diclofenac sodium in aqueous humor after ocular administration inrabbits

OBJECTIVE To establish a HPLC assay for the determination of diclofenac sodium in aqueous humor following its topical application in rabbits. METHOOS After methanol extraction,the residue was analyzed using a reversed phase C₁₈column with methanol water acetic acid(80∶20∶0.5) as the mobile phase under ultraviolet detection.The aqueous humor samples obtained from rabbits at different interval were mesured. RESULTS The good linearity was achieved over the range of 10～50 ng·mL^-1. The mean recovery of three concentrations were 99.7%.The within and between day RSD was less than 10%.Diclofenac sodium was absorbed well through the aqueous humor with the peak concentration at 1 h. CONCLUSION The method was simple, sensitive, accurate and reproducible.It should be applicable for the determination of diclofenac sodium in ocular fluids.