银杏叶提取物对AGEs诱导的大鼠HSC增殖的抑制作用

目的：探讨不同浓度晚期糖基化终产物（AGEs）对体外培养的大鼠肝星状细胞（HSC）增殖的影响，并观察不同浓度银杏叶提取物（EGb）对其增殖有无抑制作用。方法体外合成AGEs，MTT法观察不同浓度的AGEs对HSC增殖的影响及不同浓度EGb对AGEs促HSC增殖的抑制作用。结果当培养液中AGEs浓度≥50mg/L时，12～48h内检测发现HSC较正常对照组增殖明显并呈时间及剂量依赖性（P＜0.05），而6h内及低浓度AGEs组未观察到其对HSC增殖有明显影响。EGb在培养48h时对AGEs刺激的HSC的增殖有显著抑制作用（P＜0.05），且呈明显的剂量依赖性。结论 AGEs可以促进HSC增殖，呈时间、剂量依赖性；在作用时间充分的前提下，EGb可抑制AGEs诱导的HSC增殖，呈剂量依赖性。

The effect of ginko biloba extract on proliferation of hepatic stellate cell activating by advanced glycation end products

Objective To investigate the effect of ginko biloba extract （EGb） on the cell proliferation in HSC stimulated by advanced glycation end products（AGEs）. Methods AGEs was synthesized by incubating glucose with BSA in vitro. MTT colorimetric assay was used to observe the effect of AGEs at different dosages on the proliferation of HSC,and to measure the effect of EGb at different dosages on the proliferation of HSC.Results The proliferation of HSC was enhanced after incubating with ≥50mg/L AGEs for 12-48 hour with a dose and time dependent manner but within 6 hour（P〈0.05）. The low concentration of AGEs group which was not observed a significant effect on the proliferation of HSC. The proliferation of HSC was slow and exhibited a dose dependent manner with 48 hour treatment of EGb. Conclusion EGb depressed the proliferation of HSC which is induced by AGEs with a dose and time dependant manner.