CEPO调控新生大鼠缺氧缺血性脑损伤Bcl-2、Bax基因表达的研究

目的 观察新生大鼠缺氧缺血性脑损伤（HIBD）脑组织Bcl-2、Bax基因表达变化及氨基甲酰化促红细胞生成素（CEPO）干预对其表达的影响,探讨CEPO发挥脑保护作用的可能机制.方法 将新生7日龄SD大鼠建立HIBD模型,利用RT-PCR检测缺氧缺血和CEPO干预后2h、12h、24 h、48 h、72 h凋亡基因Bcl-2、Bax的mRNA表达的改变.结果 与假手术组相比,缺氧缺组在2h、12h、24 h、48 h、72 h脑组织中Bcl-2、Bax的表达均增加（P＜0.05）,与缺氧缺血组相比,CEPO干预组在不同时间点Bcl-2表达增加（P＜0.05）,Bax表达下降（P＜0.05）.结论 在新生大鼠HIBD中Bcl-2、Bax mRNA的表达发生改变,调控二者的表达水平可能是CEPO发挥脑保护的作用机制之一.

Effect of CEPO on the expression of Bcl-2 and Bax genes in hypoxic ischemic brains of neonatal

ObjectiVe To investigated the association between the expressions of Bcl-2 and Bax genes in neonatal rats upon cerebral hypoxic-isehemia and the effects of Carbamylated Erythropoietin （CEPO） administration on both genes. Methods An animal model of neonatal hypoxic-ischemia brain damage was established. The changes of Bcl-2 and Bax mRNAs in brain tissues were detected in rats after HI or CEPO administration by RT-PCR. Results The levels of Bcl-2 and Bax mRNAs were increased upon HIBD compared with sham-operated group at 2 h, 12 h, 24 h,48 h, 72 h time points post treatment （P〈0.05）. On the contrary, only the level of Bcl-2 mRNA was increased （P 〈0.05） while the level of Bax mRNA was decreased （ P 〈 0.05 ） in CEPO intervention group. Conclusion The levels of Bcl-2 and Bax mRNAs in HIBD of neonatal rats were altered. CEPO may exert some neuroprotective effects on the brain tissues via interventing the expression of Bcl-2 and Bax.