HPLC同时测定护肤粉刺霜中4种有效成分的含量

 目的：测定护肤粉刺霜中甲硝唑、氯霉素、地塞米松磷酸钠及维生素A酸的含量。方法：高效液相色谱法，以氢化可的松为内标，Nova-Pak C₁₈柱，甲醇 水为流动相；变换检测波长；梯度洗脱。结果：一次进样即可完成4种化学性质不同组分的含量分析。甲硝唑6.046 μg·ml_-1～66.51 μg·ml_-1，氯霉素5.872 μg·ml_-1～64.59 μg·ml_-1，地塞米松磷酸钠0.602 2 μg·ml_-1～6.624 μg·ml_-1，维生素A酸0.4654 μg·ml_-1～5.119 μg·ml_-1范围内，峰面积与其浓度呈良好线性关系；平均回收率依次为96.51%，RSD=1.3%；98.74%，RSD=0.9%，96.03%，RSD=1.7%，97.61%，1.9%。结论：方法简便、快速、准确，可作为样品的检测方法。

Simultaneous determination of four effective constituents in Hufufenci Shuang by HPLC

To separate and determine four constituents including metronidazole, chlormycetin, dexamethasone and vitamin A acid in Hufufenci Shuang. METHODS: A HPLC method was used. Chromatographic conditions included Nova-Pak C18 column and the mobile phase consisting of methanol and water. Hydrocortisone was used as internal standard. Alternating detection wavelength and gradient elution was used in HPLC. RESULTS: The separation and determination of four constituents having various chemical properties were accomplished by only one injecting sample. The calibration curves were linear in the range of 6.046 μg*ml-1～66.51 μg*ml-1 for metronidazole; 5.872 μg*ml-1～64.59 μg*ml-1 for chloromycetin; 0.602 2 μg*ml-1～6.624 μg*ml-1 for dexamethasone; 0.465 4 μg*ml-1～5.119 μg*ml-1 for vitamine A acid. The average recoveries of metronidazole, chloromycetin, dexamethasone and vitamin A acid were 96.51% (RSD=1.3%), 98.74% (RSD=0.9%), 96.03% (RSD=1.7%), and 97.61% (1.9%), respectively. CONCLUSION: This method was found to be simple, quick and accurate for simultaneous determination of the four constituents in Hufufenci Shuang.