葛根素对大鼠肝缺血再灌注后Caspase-3和Bcl-2表达的影响

目的：探讨葛根素对肝缺血再灌注损伤Caspase-3、Bcl-2表达及肝细胞凋亡的影响。方法：建立大鼠肝脏部分缺血再灌注模型,以葛根素预处理,免疫组化法检测Caspase-3和Bcl-2表达,透射电镜观察肝细胞凋亡的超微结构改变。结果：肝缺血再灌注后发生了显著的细胞凋亡现象,Caspase-3表达升高,Bcl-2蛋白表达减少,6h恢复正常,其后逐渐升高。葛根素预处理使细胞凋亡明显减轻,Caspase-3表达明显下降,Bcl-2持续高表达。结论：葛根素可上调Bcl-2的表达,抑制Caspase-3的表达,减少肝细胞凋亡,对肝脏缺血再灌注损伤有保护作用。

Effect of Puerarin on the Expression of Caspase-3 and Bcl-2 in Rat's Hepatic Ischemic Reperfusion Injury

Objective:To investigate the effect of puerarin on the expression of Caspase-3 and Bcl-2 and the apoptosis of hepatocytes in rat's hepatic ischemia reperfusion injury.Methods:A rat model of partial hepatic ischemia-reperfusion was found.The Caspase-3 and Bcl-2 expression were measured with immunohisto-chemical method.The apoptosis of hepatocytes were observed under electron microscope.Results:Universal hepatic cell apoptosis was found after reperfusion.The expression of Caspase-3 increased significantly,and the expression of Bcl-2 was lower at 3h after reperfusion,returned to normal level at 6h and continued to increase until 48h.When treated with puerarin,there was much less apoptosis,the expression of Caspase-3 decreased obviously and the positive Bcl-2 was significantly higher.Conclusions:Puerarin can protect the liver from the ischemia reperfusion injury by increasing Bcl-2 expression and inhibiting Caspase-3 expression,alleviating the apoptosis of hepatocytes.