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Characterization of human beta2-microglobulin by isoelectric focusing. |
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| Authors: | P W Hall E S Ricanati C V Vacca |
| Affiliation: | 1. Central Laboratory for Clinical Investigations, Osaka University Hospital, OsakaJapan;2. Endocrine Laboratory, Center for Adult Diseases, OsakaJapan |
| Abstract: | b2-Microglobulin (B2M) isolated from the urine of normal subjects and patients with cadaveric renal transplantation, showed 2 homologues by isoelectric focusing, one with a pI 5.3, the other with a pI 5.7. These proteins show identical molecular weights by dextran gel filtration and sodium dodecyl sulfate acrylamide gel electrophoresis. The immunogenic reactivity demonstrates partial identity using antiserum to human B2M from 2 different sources. |
| Keywords: | Address for correspondence and reprint requests: Kiyoshi Ichihara Central Laboratory for Clinical Investigation Osaka University Hospital 1-1-50 Fukushima Fukmhima-ku Osaka 553 Japan. |
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