益气养阴活血方对胰岛素抵抗大鼠血清纤溶酶原激活物抑制物的影响

目的观察益气养阴活血方对胰岛素抵抗模型大鼠血清纤溶酶原激活物抑制物(PAI-1)含量的影响,以初步明确其作用靶点.方法雄性Wistar大鼠随机分为空白组、模型组、中药低剂量组、高剂量组.空白组喂以普通饲料,模型组与各给药组注射小剂量链脲佐菌素并喂以高热量饲料,常规测定各组大鼠给药前后的体重、空腹血糖和给药后各组大鼠的空腹血清胰岛素水平,计算胰岛素敏感性指数,测定各组血脂、血清PAI-1水平.结果益气养阴活血方能有效地减轻模型大鼠体重,明显降低大鼠的空腹血糖和空腹胰岛素水平,改善脂代谢紊乱状况,提高胰岛素敏感指数,降低血清PAI-1水平(P＜0.05).结论益气养阴活血方对胰岛素抵抗大鼠有显著的改善作用,其机制可能与降低血清PAI-1水平有关.

Experimental Study of Yiqi Yangyin Huoxue Recipe's Effect on Serum PAI-1 Level in IR Rat

Objective:To investigate Yiqi Yangyin Huoxue Recipe`s effect on serum PAI-1 level in insulin resistance(IR) rat induced by low dose of streptozotocin(STZ) and high calorie food to find out its functional target.MethodsWTBZ]:Male Wistar rats were randomly divided into normal group,model group,low dose of Yiqi Yangyin Huoxue Recipe treating group and high dose of Yiqi Yangyin Huoxue Recipe treating group.The rats of normal group were fed with common food while the others with high calorie food and injected with low dose of STZ.The weight,fasting blood glucose(FBG),fasting insulin(FIns),blood fat,PAI-1 level of all rats were measured,and insulin sensitivity index(ISI) was calculated accordingly.Results:Yiqi Yangyin Huoxue Recipe could reduce body weight of IR rats.According to the results of biochemical detection,Yiqi Yangyin Huoxue Recipe could reduce FBG level and FIns level,improve blood fat condition,enhance ISI while reduce serum PAI-1 level effectively(P<0.05).Conclusion:Yiqi Yangyin Huoxue Recipe can ameliorate IR condition of the model rat and its mechanism is probably related to the effect of reducing serum PAI-1 secretion.