藜草花粉特异性致敏哮喘动物模型的制备

目的:建立藜草花粉粗制变应原诱导的小鼠变态反应气管炎症动物模型。方法:制备藜草花粉粗制变应原,将40只BALB/c小鼠,分为:1空白对照组、2低剂量组、3中剂量组、4高剂量组,每组10只。分别通过HE染色观察小鼠肺部炎症和黏液分泌;观察支气管肺泡灌洗液(BALF)中细胞总数和细胞分类;用酶联免疫吸附试验(ELISA)检测BALF、脾组织匀浆上清的IL-4、IFN-γ和血清抗原特异的IgE、IgG1抗体。结果:随着抗原浓度的升高肺部病理改变呈明显的变态反应炎症;BALF中的细胞总数、巨噬细胞数、EOS计数、IL-4、血清抗原特异性IgE抗体、IgG1抗体也逐步升高并明显高于空白对照组(P<0.05);BALF、脾组织匀浆上清的IFN-γ与空白对照组相比却反而下降(P<0.05)。结论:藜草花粉成功构建小鼠变态反应气道炎症动物模型。

Establishing the animal asthma model allergic to Chenopodium pollen allergen

Objective：To establish the animal asthma model by sensitizing mice in chenopodium pollen allergen.Methods：40 BALB/c mice were randomly divided into ①blank control group（n=10）②low dose group（n=10）③medium dose group（n=10）④high dose group（n=10）,after sensitization by intraperitoneal injection（i.p）and challenged by intranasal instillation of Chenopodium pollen allergen,the lung was fixed and stained with haematoxylin and eosin（HE） to evaluate the degree of proliferation of goblet cells.The total cell number and composition of BALF samples were determined.The cytokines in splenocytes culture supernatants and BALF were assayed by enzyme-linked immunoassay（ELISA）.Chenopodium pollen allergen specific IgG1,IgE in serum were also measured by ELISA.Results：Mice in model low,medium,high dose groups were not only showed inflammatory cell infiltration in airway,but also more inflammatory response with more concentration of allergen.Total cells,mononuclear,and IL-4 in BALF;allergen-specific antibody IgG1 and IgE in serum;IL-4 released from splenocytes into supernatants in vitro were all significantly increased in group low,medium,high as compared with those in group blank control（P0.05）;IFN-r in BALF and released from splenocytes into supernatants were decreased on the contrary（P0.05）.Conclusion：This model may be exploited in the study of pathophysiological and immunopathlogical mechanisms.